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Updated: Jan 9, 2026

Single Particle Cryo-Electron Microscopy: From Sample to Structure
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Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

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基于喷气玻璃化技术的微秒时间分辨率冷电磁波

Michal Haubner1, Harry M Williams1, Jakub Hruby1

  • 1Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Molecular Nanodynamics, CH- 1015 Lausanne, Switzerland.

bioRxiv : the preprint server for biology
|December 3, 2025
PubMed
概括

使用喷气玻璃化技术的新的时间分辨率冷电子显微镜 (cryo-EM) 允许科学家在几秒钟内观察蛋白质动态. 这一突破扩大了冷EM的范围.

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Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography07:00

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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