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[针对新兴传染病病原体的基于PCR的诊断试验概述]

Yu Jeong Ahn, Chaewon Jung, Jee Eun Rhee

Jugan geon-gang gwa jilbyeong

|December 3, 2025

概括

全聚合酶连锁反应 (Pan-PCR) 和多重PCR试验为新出现的传染病提供了广泛的检测. 这些PCR方法的互补使用提高了病原体的识别,并加强了公共卫生监测.

科学领域:

微生物学微生物学
分子生物学分子生物学
公共卫生公共卫生

背景情况:

病原体的持续遗传变异导致传染病爆发.
广泛的诊断技术对于检测新型和新兴传染病原体至关重要.
在COVID-19大流行之后,对先进的病原体检测的需求加剧了.

研究的目的:

审查泛聚合酶连锁反应 (泛PCR) 和多重PCR分析的研究,开发和应用.
突出它们在识别新出现或未知的传染病致病原体方面的作用.
评估它们在公共卫生监测中的互补实用性.

主要方法:

进行了全面的文献审查.
专注于最近的国内和国际报告和学术文献.
包括自COVID-19大流行以来发表的研究,强调基于PCR的诊断.

主要成果:

全PCR和多重PCR被认为是新出现的疾病的核心诊断方法.
全PCR可使用保存的基因区域对未知的病原体进行初始查.
多重PCR可以同时识别特定的病原体和共感染.

结论:

全PCR和多重PCR是积极应对传染病威胁的有希望的平台.
互补应用利用泛PCR的多功能性和多重PCR的特异性.
结合使用改善了诊断能力,并加强了对未知的病原体的公共卫生监测.

关键词:

诊断技术和程序诊断技术和程序新兴的传染病新出现的传染病.病原体是一种病原体.聚合酶连锁反应的发生.

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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...