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富血小板血治疗中的微生物污染风险和临床安全以及快速微生物检测方法的评估

Anna Arita¹, Yoshitomo Saita², Naho Fujiwara³

¹Medical Technology Innovation Center, Juntendo University, A-Bld, 3F, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.

Regenerative therapy

|December 3, 2025

概括

在丰富血小板血 (PRP) 收集和制备过程中存在微生物污染的风险. 流细胞测量显示在PRP中检测细菌有希望,但需要改进方法来确保患者的安全并预防感染.

科学领域:

微生物学微生物学
生物技术是生物技术.
临床诊断临床诊断临床诊断

背景情况:

富血小板血 (PRP) 越来越多地用于治疗目的,包括缓解疼痛和治愈伤口.
最近的报告强调了对治疗后感染的担忧,需要对与PRP相关的微生物污染风险进行调查.

研究的目的:

评估血液采集和PRP制备过程中的微生物污染风险.
评估流细胞计 (FCM) 和聚合酶链反应 (PCR) 作为PRP中微生物污染物的快速检测方法的有效性.

主要方法:

在静脉穿刺部位和PRP制备设施中评估了微生物污染风险.
在PRP和血小板贫血 (PPP) 中,FCM和PCR对于检测注射的微生物 (例如,金黄色葡萄球菌,白杆菌) 的敏感性进行了比较.

主要成果:

60%的患者和67%的制备设施在静脉穿刺部位检测到微生物殖民地.
在PRP中,FCM检测到微生物污染的度为10^2-10^3 CFU/mL,检测到*C. albicans*的局限性.
在PRP样本中,PCR显示出高的假阴性/假阳性率,尽管纯种植具有良好的灵敏度.

结论:

静脉注射和PRP生产过程存在微生物污染的风险.
建议加强消毒协议和特定设施的措施,以减轻污染.
FCM是检测PRP中可活性的细菌的一个有希望的工具,尽管需要进一步提高灵敏度. 临床医生必须对潜在的感染保持警.

关键词:

流动细胞计量流动细胞计量富含血小板血的血含量是多少聚合酶连锁反应的发生.快速微生物方法快速微生物方法再生医学是一种再生医学.

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