单细胞转录组分析分析ESWT治疗后糖尿病伤口组织的细胞动态和转录性变化
Dongyu Li1,2, Yu Wang1, Yunlong Wang3
1Department of VIP In-Patient Ward, The First Hospital of China Medical University, Shenyang, Liaoning, China.
Frontiers in physiology
|December 3, 2025
概括
身体外冲击波疗法 (ESWT) 通过重编程巨细胞,纤维细胞和内皮细胞来增强糖尿病伤口愈合. 这种非侵入性治疗促进免疫调节和组织修复,提供了一个有前途的治疗策略.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 伤口治愈研究研究 伤口治愈研究
背景情况:
- 糖尿病伤口 (DWs) 呈现出受损的愈合和有限的治疗选择.
- 身体外冲击波疗法 (ESWT) 显示出加速慢性伤口修复的潜力.
- 糖尿病伤口愈合中的ESWT细胞机制尚不清楚.
研究的目的:
- 阐明ESWT在糖尿病伤口愈合中的细胞和分子机制.
- 创建ESWT治疗的糖尿病伤口组织的综合单细胞地图.
- 确定ESWT调节的关键细胞通路,以改善组织修复.
主要方法:
- 单细胞RNA测序 (scRNA-seq) 用于分析来自糖尿病伤口组织的约39,475个细胞.
- 分析了ESWT诱导的细胞群和转录变化.
- 研究了细胞-细胞通信网络,以了解细胞间相互作用.
主要成果:
- 确定了12个主要的细胞群,包括巨细胞,纤维细胞,内皮细胞,角质细胞和免疫细胞.
- ESWT促进了修复性巨细胞扩张,激活了亲再生性纤维细胞状态,并恢复了内皮细胞血管性程序.
- ESWT减弱了促炎信号和增强了免疫细胞通信 (T细胞,NK细胞,树突细胞),促进了免疫调节和组织修复.
结论:
- 这项研究提供了ESWT诱导的糖尿病伤口变化的高分辨率单细胞地图.
- 通过调节关键细胞通路并恢复平衡的伤口微环境,ESWT增强了糖尿病伤口愈合.
- 这些发现支持ESWT作为慢性伤口管理的治疗策略的翻译潜力.
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