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来自Verticillium dahliae的双重作用GH7家族蛋白质作为毒性因子和细胞内效应因子起作用,触发NLR介导免疫力

Xiao-Bin Ji^1,2, Quan-Le Zhang², Xin-Xin Liu²

¹State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

Plant, cell & environment

|December 3, 2025

概括

来自Verticillium dahliae的真菌甘氨酸化酶7 (GH7) 蛋白质是病毒性因素,也会触发植物免疫力. 这些酶进入植物细胞,通过SGT1-NDR1通路激活防御反应.

科学领域:

植物病理学植物病理学
分子植物-微生物相互作用
菌类学菌类学是指菌类学.

背景情况:

细胞壁降解酶通常被认为是植物病原体中的细胞外毒性因素.
它们作为细胞内免疫诱导者的作用以及它们与植物免疫受体的相互作用尚未得到充分理解.

研究的目的:

研究来自Verticillium dahliae的甘氨酸酸酶7 (GH7) 蛋白在真菌毒性和植物免疫激活中的双重作用.
阐明这些蛋白与植物防御通路相互作用的机制.

主要方法:

在Verticillium dahliae中的六个GH7家族蛋白质的基因删除突变被创建来评估毒性.
使用酵母逆转酶和三甲化试验证实了蛋白质分泌.
活细胞成像,反应性氧物种爆裂试验和质沉积测量被用来研究尼科蒂亚娜·本塔米亚纳的免疫反应.

主要成果:

删除每个GH7基因显著降低了真菌毒性,但没有影响真菌生长.
证实所有六种GH7蛋白都被分泌并转移到植物细胞中,定位到细胞质中.
GH7蛋白触发了植物免疫反应,包括过敏细胞死亡,反应性氧物种爆发和质沉积,独立于它们的酶活性.
GH7触发的细胞死亡部分依赖于Skp1 (SGT1) 的G2等位基因抑制剂和非种族特异性疾病抵抗1 (NDR1) 途径,但不是增强的疾病易感性1 (EDS1).

结论:

菌GH7蛋白质具有作为毒性因子和细胞内因子的双重功能.
这些酶可以通过SGT1-NDR1信号通路激活植物的效应器触发免疫力.
这项研究为真菌代谢酶演变为效应物样分子和宿主-病原体共同演变提供了新的见解.

关键词:

欧洲资本贸易协会 (ETI)这种植物名为Verticillium dahliae.效应器效应器是一个有效的效应器.糖化酸酸酶家族7的糖化酸酸酶家族7 毒性毒性是一种毒性.

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