无症状儿童桃体组织中呼吸道病原体的分子检测:对无症状和有症状儿童之间的结果解释的影响
Amy L Leber1,2,3, Li Xu1, Sophonie J Oyeniran1,3
1Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, Ohio, USA.
Microbiology spectrum
|December 3, 2025
概括
许多儿童携带呼吸道病毒,但没有症状. 广泛的测试可以检测到这些病原体,强调需要仔细解释结果,以避免误诊.
科学领域:
- 儿童传染病 儿童传染病
- 分子诊断学 分子诊断学
- 微生物学 微生物学
背景情况:
- 核酸放大技术 (NAAT) 是诊断上呼吸道感染的标准.
- 无症状病原体携带的重要性,特别是在儿童中,往往被低估.
- 多重NAAT增加了在没有急性症状的个体中检测病原体的能力.
研究的目的:
- 调查呼吸道病原体在没有急性呼吸道感染的儿童桃体组织中的患病率.
- 评估无症状携带对儿科患者诊断解释的影响.
主要方法:
- 从522名经过桃体切除术的儿童身上分析了剩余的桃体组织.
- 使用实验室开发的聚合酶链反应 (PCR) 试验检测呼吸道病原核酸.
- 与在研究期间怀疑呼吸道感染的标准护理检测结果进行比较.
主要成果:
- 显著比例的儿童 (41.0%) 无症状地携带至少一种呼吸道病原体.
- 肠道病毒是最频繁检测到的病毒,其次是腺病毒和犀病毒.
- 同时检测多种病原体发生在5.6%的病例中,在年幼儿童中,这一比例更高.
结论:
- 呼吸道病原体经常在无症状儿童的桃体中检测到,特别是年幼的儿童.
- 这些发现强调了理解无症状携带对于准确诊断的重要性.
- 谨慎解释多重NAAT结果至关重要,以防止儿童呼吸系统疾病的误诊和过度治疗.
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