在Pichia pastoris中通过组合策略表达zearalenone lactonohydrolase ZenG的高水平表达
Qin Zhou1,2, Shida Zhao3,2, Yuzhuo Wu4
1College of Life Sciences, Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology, Key Laboratory of Xinjiang Phytomedicine Resource Utilization, Shihezi University, Ministry of Education, Shihezi, 832003, China.
Archives of microbiology
|December 3, 2025
概括
这项研究对Pichia pastoris进行了高水平的zearalenone lactonohydrolase (ZenG) 生产的工程,这种酶是排毒有害zearalenone污染的关键酶. 优化的菌株实现了显著增强的酶活性,改善了食品安全和牲畜健康.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 食品安全 食品安全
背景情况:
- 泽拉伦污染对粮食安全和牲畜健康构成重大威胁.
- 有效的排毒酶对于减轻这些风险至关重要.
- 皮奇亚牧草是一种适合复合酶生产的宿主.
研究的目的:
- 为了在Pichia pastoris.中实现zearalenone乳酸 (ZenG) 的高水平表达.
- 通过蛋白质工程和工艺改进优化ZenG活动和产量.
- 为工业规模的ZenG生产建立一个高效的平台.
主要方法:
- 在Pichia pastoris中表达的泽拉伦乳酸 (ZenG).
- 用前Ost1-pro-alpha-factor替换了α-因子信号.
- 优化基因剂量和共同表达的分子伴侣 (100p-HAC和55p-HAC1).
主要成果:
- 重组ZenG在pH8.0和50°C时表现出最佳活性.
- 信号的修改增加了ZenG活性,从180.26 U/mL增加到276.47 U/mL.
- 基因剂量优化和陪伴者共同表达进一步提高了活性,达到582.01 U/mL (3.23倍增加).
结论:
- 成功构建了一种工程P. pastoris菌株,用于高效的ZenG生产.
- 优化的菌株为工业规模应用在泽拉伦排毒中提供了坚实的基础.
- 这一进步有助于提高食品安全和畜牧业的健康.
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