对Pyruvate Decarboxylase在Saccharomyces cerevisiae中的活性进行光遗传控制,用于调乙醇生产
Meizi Liu1,2,3, Yunhong Chen1,2,4, Junjun Yan1,2,5
1Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
ACS synthetic biology
|December 3, 2025
概括
研究人员使用光遗传学设计了酵母 (Saccharomyces cerevisiae) 以控制蓝光的乙醇生产. 这种可逆的方法允许精确的,依赖于光的细胞代谢调节,克服传统基因工程的局限性.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 视觉遗传学 视觉遗传学
背景情况:
- 麦芽菌 (Saccharomyces cerevisiae) 是代谢工程的关键宿主,但表现出Crabtree效应,在高葡萄糖下有利于乙醇生产.
- 传统的代谢工程依赖于静态遗传修饰,限制了细胞过程的动态控制.
- 光遗传学为生物系统的可逆和精确时间控制提供了一个强大的工具.
研究的目的:
- 为了设计Saccharomyces cerevisiae以对代谢途径依赖光的控制.
- 开发用于动态调节乙醇生物合成的光遗传学工具.
- 为了克服Crabtree效应在酵母代谢中的局限性.
主要方法:
- 通过插入光传感器模块 (AsLOV2和cpLOV2) 来进行工程化酸盐脱碳酶异酶1 (Pdc1).
- 开发了一个基于生长表型的选系统,以识别对光敏感的酶变体.
- 创建了opto-Saccharomyces cerevisiae菌株 (MLy-9和MLy-10),用于蓝光介导的代谢控制.
主要成果:
- 确定了两种对蓝光敏感的Pdc1变体 (OptoPdc1D1和OptoPdc1D2),使其具有依赖光的酶活性.
- 在opto-S. cerevisiae菌株中证明有效调节细胞生长和乙醇生产.
- 实现了20-120倍的乙醇生物合成的动态控制范围,对光强度和脉冲模式做出反应.
结论:
- 建立了一个新的蛋白质水平策略来调节Saccharomyces cerevisiae中的代谢途径.
- 引入了一种有效的光遗传方法来精确控制乙醇代谢.
- Opto-S. cerevisiae菌株为动态代谢工程应用提供了一个可调节的平台.
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