Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Modular Assembly of Bioconjugates Enabled by a Pyridine-Based Chemoselective Sequential Conjugation Platform.

Angewandte Chemie (International ed. in English)·2026
Same author

A Sweet and Stable Strike: Multivalent Glucosylated Gd-DOTBA Conjugate with Ultrahigh Relaxivity for Targeted MRI of Aggressive Cancers.

Journal of medicinal chemistry·2026
Same author

Pyridine-Bridged Axial Coordination Creates Electron-Deficient Co-N<sub>5</sub> Porphyrin Sites for Selective Photoelectrochemical CO<sub>2</sub> Reduction on Si Nanowires.

The journal of physical chemistry letters·2026
Same author

ILDR2 impairs antitumor surveillance by recruiting immunosuppressive CCR2<sup>+</sup> monocytes.

Biochemical and biophysical research communications·2026
Same author

Driving mechanisms of adaptive strategies and heavy metal remediation potential in Artemisia lavandulaefolia during succession of abandoned Pb-Zn mining areas.

Journal of environmental management·2026
Same author

Deciphering glutamine metabolic reprogramming: a novel therapeutic target ALDH18A1 in triple-negative breast cancer.

Scientific reports·2026

相关实验视频

Updated: Jan 9, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

12.8K

基于K-Sparse词典学习方法的稀疏重建,用于生物发光断层扫描.

Kang Li, Bianbian Yang, Yi Chen

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |December 3, 2025
    PubMed
    概括
    此摘要是机器生成的。

    生物发光断层扫描 (BLT) 的重建通过一种新的AFBS-DCA算法得到了改进. 这种方法提高了生物医学研究分子成像的准确性和效率.

    更多相关视频

    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.1K
    Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
    06:28

    Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

    Published on: October 7, 2014

    13.8K

    相关实验视频

    Last Updated: Jan 9, 2026

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
    12:24

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

    Published on: July 17, 2012

    12.8K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.1K
    Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
    06:28

    Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

    Published on: October 7, 2014

    13.8K

    科学领域:

    • 分子成像学分子成像学
    • 生物医学研究的研究.
    • 医学成像分析分析 医学成像分析

    背景情况:

    • 生物发光断层扫描 (BLT) 对生物医学研究至关重要,但由于其不良位置,其重建准确性面临挑战.
    • 传统的字典学习方法用于BLT重建表现出缓慢的融合,并可以卡在次优化解决方案.
    • 提高BLT重建的灵敏度和精度对于推进分子成像应用至关重要.

    研究的目的:

    • 提出加速前后分割和形函数差异算法 (AFBS-DCA) 进行增强的BLT重建.
    • 改进BLT.稀疏编码阶段的稀疏性和趋同性能.
    • 为了降低字典更新阶段对象函数的复杂性,以实现更高效的BLT.

    主要方法:

    • 拟议的AFBS-DCA方法整合了通用最小- (GMC) 正规化和Nesterov加速,以改进稀疏编码.
    • 为了适应性调节参数调整,引入了一个k-稀疏性策略.
    • 在字典更新阶段使用差异凸函数 (DCA) 算法来简化目标函数.

    主要成果:

    • 数字模拟表明,AFBS-DCA方法显著优于传统的重建技术.
    • 拟议的方法显示了BLT.在定位精度和形状恢复能力方面的卓越表现.
    • AFBS-DCA提高了BLT重建的效率和准确性.

    结论:

    • AFBS-DCA算法在BLT重建方面取得了重大进展,解决了传统方法的局限性.
    • 这种增强的BLT重建技术为了解疾病机制和评估药物疗效提供了新的途径.
    • 提高AFBS-DCA的准确性和效率对临床前和临床分子成像研究具有前途意义.