在食品级的乳酸菌中,水性驱动的界面行为:对自然表面变异和物理化学决定因素的交叉验证
Ping Yin1, Xiaoyi Jiang1, Ying Wang1
1College of Food Science andEngineering, Nanjing University of Finance and Economics/CollaborativeInnovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China.
Colloids and surfaces. B, Biointerfaces
|December 3, 2025
概括
这项研究使用多种技术系统地描述了乳酸菌的表面特性,揭示了疏水性,表面化学和亲生菌发展的自聚合之间的相关性.
科学领域:
- 微生物学 微生物学
- 表面化学 表面化学
- 生物物理学的生物物理.
背景情况:
- 关于乳酸细菌 (LAB) 表面特性的传统研究通常使用孤立测量.
- 在LAB中关联多个表面特征的系统研究有限.
研究的目的:
- 进行对乳酸菌表面化学的综合调查.
- 通过将分子分析和物理化学验证相结合,系统地描述LAB表面特性.
主要方法:
- 根据泽塔潜力和疏水性对42种乳酸菌株的查.
- 使用富里埃变换红外光谱 (FTIR) 和X射线光电子光谱 (XPS) 对八种选定的菌株进行了深入分析.
- 物理化学验证包括水接触角度测量 (CAM) 和微生物对溶剂的粘附 (MATS).
主要成果:
- 厌水菌株的N/C比率更高 (蛋白质丰富),而爱水菌株的O/C比率更高 (多糖占主导地位).
- 对于疏水性,CAM和MATS的相关性很好,但对于易斯酸性质却有所不同.
- 高度疏水的菌株表现出更大的自聚,受泽塔潜力的影响.
结论:
- 综合的多技术策略对于全面的乳杆菌表面表征至关重要.
- 了解表面特性为益生菌和生物界面应用提供了对Lactobacillus功能的洞察.
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