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在"杜利"梨 (Pyrusbetulifolia Bunge) 中,PbNAC155通过ABA信号通路赋予盐应激耐受性

Rui Liu¹, Ziyi Li¹, Qingnan Li¹

¹College of Horticulture, Hebei Agricultural University, Baoding, 071001, China; Engineering Research Center for Pear Industry Technology, Ministry of Education, Baoding, 071001, Hebei Province, China; Pear Technology Innovation Center of Hebei Province, Baoding, 071001, Hebei Province, China.

Plant physiology and biochemistry : PPB

|December 3, 2025

概括

梨NAC转录因子,如PbNAC155,通过与ABA信号元件相互作用来增强盐分耐受性. 这项研究揭示了植物适应盐压力的新调节机制.

科学领域:

植物分子生物学植物分子生物学
无生物应激反应的应激反应
植物遗传学植物遗传学

背景情况:

NAC转录因子对于植物发育和应激适应至关重要.
植物盐应激反应中NAC基因的特定作用尚未完全理解.
梨 (Pyrus betulifolia Bunge) 是面临环境挑战的重要水果作物.

研究的目的:

为了识别和表征中涉及盐应激反应的NAC基因.
阐明PbNAC155在调节盐分耐受性的分子机制.
研究PbNAC155与其他信号通路的相互作用.

主要方法:

在Pyrus betulifolia中全基因组识别NAC基因.
在盐和ABA治疗下进行基因表达分析.
通过在梨中过度表达和淘汰PbNAC155的功能分析.
蛋白质与蛋白质相互作用试验 (Y2H,BiFC).
促进剂结合试验 (Y1H).

主要成果:

在梨子基因组中发现了178个PbNAC基因.
盐和ABA诱导了PbNAC155的表达.
过度表达PbNAC155增强了盐分耐受性,而敲击增加了敏感性.
PbNAC155与PbABI5相互作用,这是一个关键的ABA信号调节器.
PbNAC155与PbMYB88促进体结合,激活其表达.

结论:

PbNAC155-PbABI5复合体在协同调节梨的盐耐受性方面发挥着至关重要的作用.
一个涉及PbNAC155,PbABI5和PbMYB88的新型调节模块被确定用于盐应激适应.
这项研究阐明了一种新的ABA信号通路机制,用于增强植物对盐的耐受性.

关键词:

在 ABA 信号中使用 ABA 信号.在PbABI5中,PbABI5被认为是PbABI5.这就是PbNAC1555.盐的压力是盐的压力.杜丽梨是一种珍珠.

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