玉米ZmEREB199转录因子负面调节植物盐耐受性
Haoqiang Yu1, Xiaolong Lin1, Didi Lina1
1Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Plant physiology and biochemistry : PPB
|December 3, 2025
概括
土壤盐化限制了作物产量. 玉米ZmEREB199基因,当过度表达时,通过损害生长和生化反应,降低植物的盐耐受性,突出其在压力调节中的作用.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 土壤盐化对全球作物生产率构成重大威胁.
- AP2/EREBP转录因子对于植物发育和应激反应至关重要.
- 在玉米盐应激反应中DREB亚类的具体作用尚不清楚.
研究的目的:
- 为了克隆和表征玉米ZmEREB199基因.
- 研究ZmEREB199在盐应激耐受性中的表达模式和功能作用.
- 阐明ZmEREB199在玉米对盐应激反应中的调节机制.
主要方法:
- 基因克隆,序列分析,亚细胞定位和转录激活试验.
- 定量实时PCR (RT-qPCR) 用于表达式分析.
- 在阿拉比多普西斯和大米中进行转基因研究,RNA测序 (RNA-seq) 和双露西法酶记者测试.
主要成果:
- ZmEREB199编码了一种具有转录激活活性的核局部蛋白质,属于DREB亚家族.
- 玉米中的盐应激显著诱导ZmEREB199的表达.
- 过度表达ZmEREB199会损害转基因植物的盐分耐受性,由减少生长,叶绿素含量,RWC,SOD/POD活性以及增加REC,MDA,H2O2和氧化损伤标志物证明.
结论:
- ZmEREB199对玉米的盐应激耐受性进行负面调节.
- 该基因通过控制应激反应基因的转录来调节盐分耐受性.
- 这项研究揭示了ZmEREB199在玉米盐应激反应中的功能和调节机制.
更多相关视频
08:27Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
Published on: November 30, 2022
5.1K
10:28Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
24.6K
相关概念视频
Responses to Salt Stress
14.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.4K
Riboswitches
9.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.5K
Responses to Heat and Cold Stress
14.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.6K
Adaptations that Reduce Water Loss
27.8K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
27.8K
Gene Regulation During Sporulation
408
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
408
Responses to Drought and Flooding
11.9K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
11.9K
