微生物代谢物塑造了哺乳动物蛋白质的翻译
Francesca Tuorto1, Frank Lyko2
1Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Mannheim Cancer Center (MCC), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
Cell metabolism
|December 3, 2025
概括
微生物队列对于真核tRNA修饰至关重要. 一项新的研究显示,一种细菌中间体,即pre-queuosine 1 (preQ1),与queuine竞争,影响蛋白质翻译和细胞生长.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- 转移RNA (tRNA) 中的真核素queuosine修饰对于细胞功能至关重要.
- 这种修饰依赖于分子队列的吸收,主要来自微生物来源.
- 微生物代谢物影响宿主细胞过程的精确机制是一个活跃的研究领域.
研究的目的:
- 研究细菌队列生物合成中间体在哺乳动物细胞中的作用.
- 阐明在tRNA修饰中涉及的pre-queuosine 1 (preQ1) 和宿主细胞标之间的相互作用.
- 了解这种相互作用如何影响基本的细胞过程,如蛋白质翻译和增殖.
主要方法:
- 利用生物化学分析来研究preQ1,queuine和哺乳动物tRNA之间的结合相互作用.
- 在不同的条件下使用基于细胞的测试来监测蛋白质翻译速率和细胞增殖.
- 分析了preQ1和队列对特定tRNA修饰途径的影响.
主要成果:
- 证明了pre-queuosine 1 (preQ1),一种细菌队列生物合成中间体,直接与队列在与哺乳动物tRNA标结合方面竞争.
- 表明这种竞争对抗性地调节蛋白质翻译效率.
- 观察到preQ1影响哺乳动物细胞增殖,这表明它对宿主细胞生理有重大影响.
结论:
- 前queuosine 1 (preQ1) 作为一种关键的微生物代谢物,可以干扰宿主细胞tRNA修饰.
- 预Q1和队列之间的竞争突出了影响蛋白质合成和细胞生长的宿主微生物相互作用的新机制.
- 这一发现有助于理解肠道微生物群对宿主细胞功能和生物体健康的更广泛影响.
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