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开发和应用一个带条码的狂犬病病毒追踪方法,用于映射整个大脑输入到单个神经元的输入

Kang Tan¹, Ya-Qian Wang², Rong-Rong Yang³

¹Institute of Neuroscience and State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China; Lingang Laboratory, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, 555 Qiangye Road, Shanghai 201210, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Cell reports methods

|December 3, 2025

概括

研究人员开发了条码狂犬病病毒追踪 (BRT) 来绘制神经输入连接的地图. 这种方法揭示了局部和远程输入之间的关系,有助于大脑连接体的构建.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
计算生物学计算生物学

背景情况:

绘制神经连接的地图对于理解大脑电路至关重要.
目前的方法很难为输入映射提供单细胞分辨率.

研究的目的:

开发一种用于映射单个神经元的输入连接的新方法.
分析介质前额叶皮层中局部和远程输入之间的关系.

主要方法:

结合逆向病毒追踪与单细胞RNA测序.
开发了带条码的狂犬病病毒追踪 (BRT) 用于高分辨率的输入组映射.
在小鼠中部前额叶皮层施加BRT.

主要成果:

BRT成功地将本地和远程输入连接映射到转录组定义的神经元.
观察到单个神经元接收的输入数量的变化.
发现本地输入数量与远程输入区域之间存在正相关性.

结论:

BRT是一种强大的工具,用于在细胞水平上构建详细的大脑连接体.
输入组复杂性受到远程神经输入的多样性的影响.
这种方法为中观连接体构造提供了基础.

关键词:

在CP:神经科学.条形码狂犬病病毒追踪病毒追踪高度内化的神经元的神经元.中部前额叶皮层中部前额叶皮层单个神经元输入连接的输入连接.启动电池干扰的干扰

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