对温度敏感的OfARF6通过auxin反和蛋白质降解来控制Osmanthus fragrans的开花
Shiwei Zhong1,2, Wenle Li1, Boshi Fang1
1Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, College of Landscape and Architecture, Zhejiang A&F University, Hangzhou, Zhejiang, China, 311300.
The Plant journal : for cell and molecular biology
|December 3, 2025
概括
低温可以通过调节auxin水平来促进Osmanthus fragrans的开花. 一种特定的蛋白质OfARF6维持了auxin平衡,并控制了开花,将温度与植物繁殖联系起来.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 花开对于植物繁殖至关重要,受温度和内部信号通路等环境因素的影响.
- 在Osmanthus fragrans中,环境温度 (AT) 和auxin信号是开花的关键调节者.
- 之前的研究表明,低AT通过增加auxin水平来促进开花.
研究的目的:
- 为了研究奥斯曼修斯芳香的开花中奥克辛度的确切作用.
- 阐明连接温度,辅酶信号和花朵发育的分子机制.
- 为了确定关键的调节蛋白涉及温度依赖的花开和auxin平衡.
主要方法:
- 在不同度的辅素下,花开的分析.
- 研究OfARF6蛋白质的温度依赖激活和辅酶调节的稳定性.
- 对OfARF6过度表达效应的转录组分析.
- 蛋白质与蛋白质相互作用研究 (OfARF6和OfBPEub).
- 在异质系统 () 中对OfARF6的功能验证.
主要成果:
- 在O. fragrans中,花开发生在特定的,狭窄的auxin度范围内;过度的auxin抑制了这一过程.
- 通过蛋白质酶介导的降解,ARF6蛋白质的稳定性受到auxin水平的调节,其激活取决于温度.
- OfARF6与OfBPEub相互作用,增强细胞扩张基因 (例如,OfEXP15-4) 的转录,将温度线索与辅酶信号连接起来.
- 在花中过度表达OfARF6降低了auxin水平和损害了花开,证实了其在auxin恒温中的作用.
结论:
- 在O. fragrans.中,OfARF6充当一个关键的节点,将温度信号与auxin稳态结合起来,以调节花开.
- 一个涉及OfARF6活性,auxin水平和EXP/PIP基因转录的负反循环维持了开花的稳态.
- 这些发现为植物对温度敏感的开花和辅酶调节的分子基础提供了新的见解.
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