通过以热酸盐为基础的固体发酵,高效地从林氏纤维素化物中生产epsilon-poly-l-lysine
Xin Zeng1, Siyu Tong1, Qiran Diao2
1Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Huaibei Normal University, Huaibei 235000, China; School of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
Bioresource technology
|December 3, 2025
概括
一种新的固态发酵 (SSF) 策略显著提高了西隆-多聚-l-氨酸 (ε-PL) 的生产. 这种方法使用基纤维素解酸盐和化物,比传统的浸泡发酵 (SmF) 获得更高的度和生产率.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物化学工程 生物化学工程
背景情况:
- 用于生产epsilon-poly-l-lysine (ε-PL) 的水下发酵 (SmF) 面临诸多挑战,包括高能耗,废水产生和氧气转移不佳.
- 这些局限性阻碍了有效和可持续的工业规模的e-PL生物合成.
研究的目的:
- 开发一种改进的e-PL生产方法,解决SmF的缺点.
- 为了研究一种新的固态发酵 (SSF) 策略的有效性,该策略利用基纤维素化物和化物用于 ε-PL 生物合成.
主要方法:
- 实施了一种固态发酵 (SSF) 策略,使用纤维素酸化物作为基质,使用化物作为固体载体.
- 优化了种植条件,包括子注射,初始pH值 (6.5),中等和率 (90%),并在7天内采用了原子化养系统.
- 分析了关键基因转录,酶活性,能量代谢和前体池,以了解生产增强.
主要成果:
- 与传统的SmF相比,达到47.6g/L的最大e-PL标位,生产率增加1.88倍.
- 观察到增强的基因表达,酶活性,能量代谢和前体可用性.
- 通过直接传递氧气,在现场去除产品/抑制剂以及降低氨毒性来促进.
结论:
- 开发的SSF战略提供了一种高效和潜在的可持续方法来生产e-PL.
- 这种方法克服了SmF的关键局限性,为工业规模的e-PL生物合成铺平了道路.
- 这些发现突显了在先进的发酵过程中,纤维素资源和烯酸的潜力.
相关概念视频
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...


