神经FF通过促进草 (Ctenopharyngodon idella) 的肠道屏障功能来增强抗菌防御
Pingyuan Li1, Jiaxin Liu2, Jiayi Hu1
1Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China.
Fish & shellfish immunology
|December 3, 2025
概括
神经FF (NPFF) 增强了草的肠壁完整性和抗菌免疫力. 这一发现为在水产养殖中管理鱼类疾病提供了潜力.
科学领域:
- * 鱼类免疫学 鱼类免疫学
- * 神经内分泌学 * 神经内分泌学
- * 粘膜免疫系统的免疫力
背景情况:
- * 肠道屏障对于鱼类对病原体的防御至关重要.
- *鱼类肠道屏障的神经免疫调节,特别是涉及神经的研究不足.
- *已知神经FF (NPFF) 能影响哺乳动物的免疫功能,但其在teleost粘膜免疫中的作用尚不清楚.
研究的目的:
- * 研究神经FF (NPFF) 在草 (Ctenopharyngodon idella) 的肠道免疫中的功能.
- * 探索NPFF对肠道屏障完整性,免疫基因表达和抗菌防御的影响.
主要方法:
- * 在草组织中对NPFF的基因表达分析.
- * 在实验室中使用复合草NPFF对上皮细胞的研究.
- * 在体内给予NPFF以评估对肠道屏障功能,免疫反应和Aeromonas hydrophila挑战后的细菌负载的影响.
主要成果:
- *在草鱼组织中检测到NPFF基因表达,在细菌挑战后,肠道,脏和脏的升调.
- *复合NPFF促进了上皮细胞的增殖和生存在体外.
- * 在体内给予NPFF,通过上调密集结蛋白 (ZO-1,Occludin,Claudin-1),增加MUC2,免疫调节因子 (AhR,IL-22) 和抗菌 (LEAP-2,Lyz1,Hepcidin-1) 来增强肠道屏障完整性,从而减少细菌负荷和改善生存率.
结论:
- *NPFF在维持肠道屏障功能和增强草的抗菌免疫力方面发挥着重要作用.
- * NPFF的使用有效地增强了鱼类的粘膜免疫力和抗病能力.
- * NPFF为改善水产养殖中的鱼类健康和疾病管理提供了一个有希望的治疗目标.
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