相关实验视频

Updated: Jan 9, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

m⁶A从核糖体结合的mRNA进行免疫沉

Nora T Kiledjian¹, Morgan J McGrath¹, Crystal S Conn¹

¹Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.

Methods in enzymology

|December 3, 2025

概括

这项研究引入了一项新协议,将RNA的翻译水平与其N6-甲基氨酸 (m⁶A) 状态联系起来. 该方法结合了多体形和m⁶A免疫沉 (IP) 进行综合分析.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
在RNA生物学,RNA生物学.

背景情况:

N6-甲基氨酸 (m6A) 是一种普遍存在的RNA修饰,影响RNA稳定性,局部化和拼接.
m⁶A对转化选择性的影响是一个活跃的研究和辩论领域.
现有的方法缺乏全面的方法,可以同时评估跨成文的翻译状态和m⁶A修改水平.

研究的目的:

开发和介绍一个强大的协议,用于将转录翻译状态与m⁶A修改相关联.
为了能够广泛地描述m⁶A存在和翻译程度之间的关系.
为研究m⁶A在基因表达调节中的功能作用提供一种工具.

主要方法:

利用多体形来分离基于核糖体占用率的RNA,作为转化活动的代理.
采用免疫沉 (IP) 专门分离含有m6A修饰的RNA分子.
集成的多元体概况与m6A IP分析跨不同转化状态的转录的m6A状态.

主要成果:

成功建立了一个协议,同时评估RNA翻译和m⁶A修改.
启用了基于其翻译效率和m⁶A内容的转录的隔离和表征.
提供了进一步研究的基础,探索m⁶A在翻译控制中的功能影响.

结论:

开发的协议提供了一个强大的方法来研究RNA修饰和翻译之间的相互作用.
这种方法有助于更深入地了解m⁶A如何在转化层面影响基因表达.
这些发现为剖析m⁶A在各种生物过程中的调节作用铺平了道路.

关键词:

史诗转录组学史诗转录组学 N6 - - 甲基亚诺辛的使用.多种群的概况分析.蛋白质合成蛋白质合成基因组RNA的修饰是RNA的修饰.m(6) 一种免疫沉.在mRNA的翻译过程中,mRNA的翻译

更多相关视频

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

相关概念视频

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...