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阿斯普鲁索胺通过降低ATF6信号通路和炎症因子的调节来抑制膀癌细胞迁移和增殖

Li Wang¹, Fei Zhang¹, Jiaren Pan¹

¹Department of Urology, Ningbo No. 2 Hospital, 315000 Ningbo, Zhejiang, China.

Archivos espanoles de urologia

|December 3, 2025

概括

阿斯珀化 (ASP) 通过降低激活转录因子6 (ATF6) 信号的调节,有效地抑制膀癌 (BLCA) 细胞的增殖,迁移和侵入. 这种天然化合物还可以减少炎症,并改变表皮细胞-介质细胞转换 (EMT) 标记物,显示出治疗潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

膀癌 (BLCA) 是一个重要的健康问题,具有复杂的分子基础.
激活转录因子6 (ATF6) 信号与癌症进展有关,包括BLCA.
天然化合物正在研究其潜在的抗癌特性.

研究的目的:

研究阿斯鲁化物 (ASP) 对膀癌 (BLCA) 细胞增殖,侵袭和上皮-介质细胞过渡 (EMT) 的抑制作用.
为了确定ASP是否通过降低激活转录因子6 (ATF6) 信号的调节来发挥其作用.
评估ASP对BLCA细胞中炎症标记物的影响.

主要方法:

在BLCA组织和正常邻近组织中评估ATF6和EMT标志物表达.
使用T24 BLCA细胞进行体外实验,包括转染,ASP治疗,细胞生长,细胞亡 (TUNEL试验),迁移,入侵 (Transwell试验) 以及对EMT和炎症标志物的西部斑块/qRT-PCR分析 (IL-6,CCL2,CXCL10).
通过检查其过度表达的影响来研究ATF6的作用.

主要成果:

在BLCA组织中观察到较高的ATF6水平与正常组织相比 (p < 0.01).
在T24细胞中,ASP治疗剂量取决于细胞侵入和迁移的减少 (p < 0.05) 和细胞亡的增加 (p < 0.05).
ASP调节的EMT标志物 (上调的E-cadherin,下调的N-cadherin和Snail) 和降低的炎症性细胞因子mRNA水平 (IL-6,CCL2,CXCL10) (p <0.01). 过度表达ATF6部分扭转了这些影响.

结论:

阿斯珀化物 (ASP) 抑制了T24膀癌 (BLCA) 细胞中的激活转录因子6 (ATF6) 信号,抑制了亡抵抗,迁移和炎症基因表达.
ASP表现出多目标能力,通过向内质网膜 (ER) 应力-ATF6轴,同时影响炎症,瘤增殖和转移.
ASP是用于BLCA治疗的有前途的天然化合物,与传统的单剂相比,它具有潜在的优势.

关键词:

激活转录因子6 (ATF6) 的亚斯鲁化物 (ASP) 是一种膀癌 (BLCA) 是一种癌症.细胞迁移细胞迁移细胞的增殖细胞的增殖.

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