蜂通过促进氧化损伤和破坏酵母模型中的宏分子组成来损害细胞增殖
Berna Kavakcıoğlu Yardımcı1,2, Nihal Şimşek Özek3,4, Ayşenur Güler5
1Department of Chemistry, Faculty of Science, Pamukkale University, Denizli, Türkiye.
Scientific reports
|December 3, 2025
概括
蜂对酵母细胞表现出双重作用,在低度时作为无毒剂,但在较高剂量时诱导细胞损伤和氧化应激. 剂量对于确定的安全性和有效性至关重要.
科学领域:
- *药理学和毒理学 *药理学和毒理学
- * 细胞生物学 细胞生物学
- * 菌群学 * 菌群学
背景情况:
- *蜂是一种来自蜜蜂的树脂,具有多种药理学活性.
- *其对细胞平衡的剂量依赖性影响尚不清楚.
- *安纳托利亚提取物被用于研究毒理效应.
研究的目的:
- * 评估标准化安纳托利亚提取物对Saccharomyces cerevisiae的毒理作用.
- * 确定子对酵母细胞平衡的度依赖作用.
- * 为了确定石诱导的氧化应激和细胞损伤的门.
主要方法:
- * 24小时内将野生类型酵母和删除突变体暴露在提取物 (0.005-0.125%v/v) 中.
- *细胞增殖测定,FTIR光谱和细胞表面完整性的评估.
- * 测量总氧化剂状态 (TOS),总抗氧化剂状态,脂质过氧化,蛋白质和DNA损伤.
主要成果:
- * прополис在0.01% (v/v) 之前是无毒的,但在≥0.025% (v/v) 时增殖率降低.
- *较低度 (0.01%v/v) 损害了突变菌株比野生类型更多.
- *氧化应激 (TOS增加) 从0.01% (v/v) 发生,在较高度下损害脂质,蛋白质和DNA.
结论:
- * 石在对酵母细胞的影响中表现出度依赖的二分法.
- *剂量是 прополис安全性及其诱导氧化应激的潜力的一个关键决定因素.
- * 该研究强调了基应用剂量标准化的重要性.
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