综合分析揭示了口腔癌进展期间的极性蛋白失调
Sandip Ghose1, U Renuka Chaudry2, Shouvik Chakravarty3
1Department of Oral Pathology, Dr R Ahmed Dental College & Hospital, Kolkata, West Bengal, India. sanindra1967@gmail.com.
Scientific reports
|December 3, 2025
概括
表皮细胞极性调节器PAR3,SCRIBBLE和DLG7在口腔癌前病变和口腔癌中显著减少. 这种极性蛋白质的丧失表明了口腔瘤发生的早期分子事件,并表明了早期检测的潜在生物标志物.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 分子病理学分子病理学
背景情况:
- 口腔癌前病变的恶性转变涉及上皮细胞极性和上皮-介质细胞过渡 (EMT) 的破坏.
- 了解极性调节者的作用对于识别口腔致癌的早期分子事件至关重要.
研究的目的:
- 调查极性调节器 PAR3,SCRIBBLE 和 DLG7 在正常口腔粘膜 (NOM),口腔上皮质发育不良 (OED) 和口腔状细胞癌 (OSCC) 中的差异表达.
- 阐明极性破坏作为口腔瘤发生的驱动机制的作用.
主要方法:
- 从习惯性烟草使用者的FFPE组织样本中进行了组织病理学评估和H&E染色.
- 对PAR3,SCRIBBLE和DLG7表达和定位模式的免疫组织化学 (IHC) 分析.
- 来自OED和OSCC患者的活检样本的全转录组RNA测序.
主要成果:
- 与NOM相比,OED和OSCC观察到PAR3,SCRIBBLE和DLG7表达的显著减少或完全丧失.
- 从NOM到OED,表皮质极性蛋白表达的显著下降,随后在OSCC中出现了适度的复苏.
- IHC和转录基因特征 (DLG7除外) 之间强烈的一致性突显了口腔致癌的早期极性破坏.
结论:
- 极性调节器PAR3,SCRIBBLE和DLG7在口腔上皮质发育不良和口腔癌中显著下调.
- 细胞极性的破坏是口腔致癌的早期和中心分子事件.
- PAR3,SCRIBBLE和DLG7作为早期检测口腔潜在恶性疾病 (OPMDs) 中恶性转变的生物标志物具有前景.
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