在犬乳腺腺癌中改变了microRNA配置文件和相关途径
Mykhailo Huniadi1, Natalia Nosalova1, Lubica Hornakova1
1Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenskeho 73, Kosice, 041 81, Slovak Republic.
Scientific reports
|December 3, 2025
概括
狗乳腺瘤是人类乳腺癌的一个模型,在腺癌中显示出改变的microRNA (miRNA) 水平. 这些特定的miRNA变化与癌症途径有关,表明对犬类和人类癌症的潜在诊断和治疗生物标志物.
科学领域:
- 比较性瘤学研究
- 分子生物学分子生物学
- 生物医学研究的研究.
背景情况:
- 狗乳腺瘤 (CMTs) 在雌性狗中普遍存在,并作为人类乳腺癌 (BC) 研究的有价值模型.
- 乳腺腺癌 (AMG) 是CMT常见的和激进的亚型,需要进一步调查其分子基础.
研究的目的:
- 与健康组织相比,分析犬乳腺腺癌 (AMG) 中的微RNA (miRNA) 表达特征.
- 确定在犬类AMG中放松调节的特定miRNA,并探索它们与关键瘤性途径的关联.
- 评估这些miRNAs作为CMT诊断和治疗生物标记物的潜力.
主要方法:
- 量化逆转录聚合酶链反应 (qRT-PCR) 用于描述犬类AMG和健康乳腺组织中84个miRNA的表达.
- 进行了生物信息通路丰富分析,以将放松调节的miRNA与相关的瘤信号网络联系起来.
主要成果:
- 在犬类AMG中,MiRNA分析显示了miR-101,miR-106b,miR-143,miR-15a,miR-205和miR-93的显著上调.
- 相反,在狗的AMG组织中,let-7c,miR-10b,miR-191和miR-26a被发现是下调的.
- 丰富的途径包括PI3K/AKT/mTOR,Wnt/β-catenin和上皮细胞-介质细胞过渡 (EMT) 调节,这表明与人类BC保持机制.
结论:
- 该研究确定了与犬乳腺腺腺癌相关的特定miRNA表达特征.
- 这些发现突出了犬类和人类乳腺癌之间保存的分子机制,加强了CMT作为模型的实用性.
- 狗AMG中的放松调节的miRNAs显示出作为潜在的生物标志物,用于诊断和治疗策略在狗和人类的癌症.
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