对比生理和转录基因分析揭示了关键的代谢途径,涉及到果在成熟过程中参与纳古梨的乙醇发酵
Sijie Dong1, Jingyi Lv1, Xin Sun1
1College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, PR China.
Journal of agricultural and food chemistry
|December 4, 2025
概括
瓜梨由于与粉降解和特定酶活动相关的较高乙醇发酵而产生"酒精味道". 这项研究确定了关键的代谢途径和调节梨中这种独特风味化合物的基因.
科学领域:
- 植物生理学 植物生理学
- 水果成熟的生物化学 果实成熟的生物化学
- 代谢学 代谢学 代谢学
背景情况:
- 果梨表现出一个独特的不同.
- 有酒精味道的酒精味道.
- 在成熟过程中,与京贝梨不同.
- 了解这种味道的生物化学基础对于水果质量研究至关重要.
研究的目的:
- 研究Nangao和Jingbai梨之间与乙醇发酵相关的代谢差异.
- 阐明底层的监管机制.
- 有酒精味道的酒精味道.
- 在Nangao梨中的形成.
主要方法:
- 对酶活性 (pyruvate脱碳酶,酒精脱酶) 和代谢物水平 (pyruvate,乙,乙醇,乙-CoA,酸) 的比较分析.
- 测量粉降解,呼吸速度和ATP水平.
- 转录组分析以确定协同调节途径和关键基因表达.
主要成果:
- 瓜梨显示出较高的酸盐脱碳酶 (PDC) 和酒精脱酶 (ADH) 活动,并增加了酸盐,乙和乙醇含量.
- 在Nangao梨中观察到高粉降解,乙-CoA和酸水平,以及下呼吸和ATP.
- 转录组数据表明,乙醇代谢通过粉转化为糖,糖解,TCA循环和酸途径进行协同调节.
结论:
- 乙醇的积累和乙醇的积累
- 有酒精味道的酒精味道.
- 在Nangao,梨与增强的发酵途径有关.
- 包括PuBMY1,PuPDC1,PuHK3,PuPFK1和PuG6PDH1在内的特定基因与初始乙醇积累相关.
- 调查结果为管理监管网络提供了洞察力.
- 有酒精味道的酒精味道.
- 在Nangao梨中.
更多相关视频
相关概念视频
01:29Fermentation
128.5K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
128.5K
01:20Fates of Pyruvate
10.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.4K
01:40Overview of Metabolism
37.5K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
37.5K