相关实验视频

Updated: Jan 9, 2026

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

米SINA E3链酶二分法控制ERECTA1的无处不在和稳定性,以调节形态和谷物产量

Zi-Qi Lu¹, Tao Guo², Ying-Jie Cao³

¹State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

Molecular plant

|December 4, 2025

概括

米的发育受到OsER1.1的调节. 缺少米中的七个 (SINAR) E3链酶控制OsER1的稳定性,影响谷物产量. 操纵SINAR酶可以通过优化OsER1信号来提高大米产量.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

米的结构对谷物产量至关重要,受受体类激酶OsER1.1的影响.
目前尚不完全了解OsER1微调恐慌发展的精确机制.

研究的目的:

为了阐明SEVEN IN ABSENTIA OF RICE (SINAR) E3泛酸酶家族在调节米粒结构中的作用.
研究SINAR酶如何调节OsER1的稳定性和信号,以影响谷物产量.

主要方法:

在大米中研究了SINAR E3酶家族成员的相互作用和功能.
分析了由SINAR连接酶调解的OsER1的泛化和降解.
评估了操纵SINAR结合酶活性对水发育和谷物产量的影响.

主要成果:

SINAR E3链酶通过协同或对抗调节OsER1的无处不在和稳定性来调节大米尖的数量.
SINAR1和SINAR6作为分子制动剂,形成异构体来抑制SINAR2/3/4/5的泛化活性,从而保护OsER1免受降解.
针对性抑制SINAR1和SINAR6通过促进OsER1降解来提高谷物产量,优化了没有损害生育能力的形结构.

结论:

SINAR1和SINAR6是负反循环的关键组件,确保了最佳的OsER1信号和恐慌架构.
一种对抗性,酶介导的调节机制维持了OsER1受体的稳态.
操纵二分化的SINAR E3酶提供了一种提高大米产量的策略.

关键词:

在OSER1中.这就是SINARs.反对性的合酶.恐慌式建筑的架构米米饭米饭米饭米饭.

更多相关视频

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

相关概念视频

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

The Unfolded Protein Response

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...

Small GTPases - Ras and Rho

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...