转录因子NtWRKY75通过协调尼古提纳烟草中的硫化平衡,加速暗感叶子衰老
Songchong Lu1,2, Yan Sun1, Hao Wu2
1Shandong Key Laboratory for Germplasm Innovation of Saline-Alkaline Tolerant Grasses and Trees, College of Grassland Science, Qingdao Agricultural University, Qingdao, 266109, China.
The Plant journal : for cell and molecular biology
|December 4, 2025
概括
烟草叶的衰老是由NtWRKY75加速的,这是一个转录因子,它负面调节硫化 (H2S) 生物合成基因. 这就是WRKY因子.
科学领域:
- 植物生物学 植物生物学
- 分子植物生理学分子植物生理学
- 生物化学 生物化学
背景情况:
- 叶子衰老是营养回收的关键发展阶段,受硫化 (H2S) 等信号分子的影响.
- 控制H2S水平的精确分子机制及其在烟草叶衰老中的作用仍然不完全理解.
研究的目的:
- 阐明WRKY转录因子NtWRKY75在调节H2S生物合成中的作用及其对烟草叶子衰老的影响.
- 为了确定关键的基因和参与NtWRKY75介导的衰老途径中的蛋白质相互作用.
主要方法:
- 对NtWRKY75,H2S生物合成基因 (NtLCD1,NtDCD1),NtOASA1和氨基酸载体 (NtAAP3) 的基因表达分析 (转录水平).
- 产生和分析过度表达NtWRKY75和CRISPR/Cas9产生的ntwrky75突变的转基因烟草植物.
- 分子和生物化学测定,包括促进体结合分析和蛋白质-蛋白质相互作用研究 (体内和体外) 与NtVQ47.
主要成果:
- 在黑暗诱导和自然叶子衰老期间,NtWRKY75的表达增加,在过度表达时加速衰老,并在突变物中延迟衰老.
- NtWRKY75直接降低了NtDCD1的调节,并提高了NtOASA1的调节,从而减少了H2S的积累.
- NtWRKY75高调氨基酸载体基因 (例如,NtAAP3) 并与NtVQ47相互作用以调节衰老.
结论:
- NtVQ47-NtWRKY75调节模块在控制H2S水平和调节烟草中的叶子衰老方面发挥着重要作用.
- 这项研究确定了关键的分子参与者和精确调节叶子衰老的途径,为作物改善提供了潜在的目标.
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