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在肺结核病中WNT信号基因甲基化的诊断潜力

Hua Wang¹, Chao-Cai Wang², Qian Huang^3,4

¹Department of tuberculosis, Anhui Chest Hospital, Hefei, Anhui, China.

Frontiers in immunology

|December 4, 2025

概括

在肺结核 (PTB) 患者中,WNT通路基因的DNA甲基化水平发生变化. 在SFRP1,WNT3A,CTNNB1,DKK-1和LRP6中甲基化降低可能作为PTB诊断的潜在生物标志物.

科学领域:

遗传学遗传学是一种遗传学.
分子生物学分子生物学
医学诊断医学诊断医学诊断

背景情况:

WNT信号通路与肺结核病理生理学有关.
WNT通路基因的异常DNA甲基化可能会影响PTB易感性和进展.

研究的目的:

研究特定WNT信号通路基因的DNA甲基化水平与PTB之间的关联.
评估这些甲基化水平作为PTB诊断生物标志物的潜力.

主要方法:

使用MethylTarget技术分析了98名PTB患者和96名对照者的血液样本中WNT通路基因 (SFRP1,WNT3A,CTNNB1,WIF-1,DKK-1,LRP5,LRP6) 的DNA甲基化水平.
评估了甲基化水平和临床特征之间的相关性.

主要成果:

与对照组相比,在PTB患者中观察到SFRP1,WNT3A,CTNNB1,DKK-1和LRP6的甲基化水平显著降低.
SFRP1甲基化与药物诱导的肝损伤和肺部感染相关;WNT3A和CTNNB1甲基化与药物耐药性和发烧相关.
CTNNB1甲基化显示了最高的诊断值 (AUC=0.706),组合面板提供轻微改善 (AUC=0.710).

结论:

减少SFRP1,WNT3A,CTNNB1,DKK-1和LRP6的DNA甲基化与PTB有关.
这些甲基化模式,单独或在面板中,显示出作为PTB的诊断生物标志物的潜力.

关键词:

通过DNA甲基化.在WNT信号通路基因.表观遗传学是指表观遗传学.肺结核 - 肺结核结核病是一种肺结核病.

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