微波辅助从枣种子中提取生物活性成分,使用深度浸泡溶剂:生物活性和抗生素辅助潜力对抗多药耐药沙门氏菌Typhimurium DT104
Hina Khan1, Priti Mudgil1, Bismi Phasaludeen1
1Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, 15551 Al Ain, United Arab Emirates.
Food chemistry: X
|December 4, 2025
概括
深度浸泡溶剂 (DES) 从日种子中提取富含多的生物活性化合物 (PBC). 这些PBC协同增强了抗生素对抗多药耐药沙门氏菌Typhimurium DT104.4.的有效性.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 绿色化学 绿色化学
背景情况:
- 抗菌素耐药性 (AMR) 是一个日益增长的全球健康威胁.
- 辅助剂可以增强抗生素活性,提供一种打击AMR的策略.
- 深度浸泡溶剂 (DES) 提供了一种可持续的方法来提取生物活性化合物.
研究的目的:
- 评估DES提取的富含多的生物活性化合物 (PBC) 对抗多药耐药沙门氏菌Typhimurium DT104.4的辅助活性.
- 评估基于DES的提取物与常规抗生素的协同作用潜力.
主要方法:
- 富含多的生物活性化合物 (PBCs) 被从枣种子中提取,使用微波辅助的多基DES.
- 福利埃变换红外光谱 (FTIR) 和超高性能液体染色学用光电极管阵列检测 (UHPLC-PDA) 用于表征.
- 进行了检查板和时间杀戮测试,以评估抗微生物和辅助活性.
主要成果:
- 基于1,4-butanediol (1,4-But) 的DES提取物表现出最高的总含量和抗氧化活性.
- 德斯提取物表现出有限的本质抗菌活性,但显著提高了抗生素疗效.
- 与ACSSuT抗生素观察到协同效应,降低它们的最小抑制度 (MIC) 高达1024倍 (FICI ≤0.5).
结论:
- 基于DES的枣提取物,特别是基于1,4-But的提取物,作为有效的辅助剂.
- 这些提取物可以显著提高抗生素对抗多药耐药性食品传播病原体的疗效,如沙门氏菌Typhimurium DT104.4.
- 这项研究强调了DES辅助提取在开发抗抗菌素耐药性的新战略方面的潜力.
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