在Enterobacteriaceae中3' UTR衍生的sRNA UhpU的起源和适应性进化轨迹
Xiaomin Chen1, Cheng Bei1, Jialei Liang1
1Key Laboratory of Medical Molecular Virology (Ministry of Education / National Health Commission / Chinese Academy of Medical Sciences), Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200033, China.
概括
来自3'未翻译区域 (UTRs) 的细菌小RNAs (sRNAs) 调节基因. UhpU,来自hexose酸盐转运基因的sRNA,从一个处理过的调节器演变为一个具有扩展连接和双重生物生成的Escherichia.
科学领域:
- 细菌遗传学 细菌遗传学
- RNA生物学的RNA生物学
- 进化基因组学是进化的基因组学.
背景情况:
- 来自mRNA 3'未翻译区域 (3' UTRs) 的细菌小RNAs (sRNAs) 是关键的基因表达调节者.
- 它们的进化路径和功能多样化比蛋白质编码序列的理解要少.
- UhpU,来自酸转运基因 (uhpT) 的sRNA,作为研究3' UTR衍生的sRNA进化的模型.
研究的目的:
- 为了比较分析Escherichia和沙门氏菌中UhpUsRNA的生物发生和调节功能.
- 阐明UhpU的进化轨迹,从其起源到其当前的监管作用.
- 调查UhpU获得新的监管目标和转录机制.
主要方法:
- 对UhpT及其相关调控元素的比较基因组分析. Enterobacteriaceae.
- 生物信息预测和sRNA处理和转录的实验验证.
- 功能性测试以确定UhpU的监管目标和机制,包括抑制色光转移酶系统基因和mprA.
主要成果:
- UhpU可能源于Enterobacteriaceae中uhpTmRNA的RNase E处理,调节酸恒温.
- 在Escherichia,UhpU通过横向基因转移和获取结合部位获得了新的监管目标mprA.
- 在uhpT编码区域内,一个特定于谱系的FliA-依赖的促进子允许在Escherichia中独立的UhpU转录,建立双重生物发生.
结论:
- UhpU呈现了一条渐进的进化路径,从处理衍生的代谢调节器到具有扩展调节连接的sRNA.
- UhpU的进化突出了横向基因转移,RNA处理和转录调节在细菌适应中的动态相互作用.
- 在Escherichia中UhpU的双重生物发生提供了一个独特的模型,用于理解单个sRNA的多个调节策略的功能和进化意义.
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