Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Diversity of Archaea II01:24

Diversity of Archaea II

429
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
429
Overview of Archaea01:29

Overview of Archaea

758
Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...
758
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

728
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
728
Diversity of Archaea IV01:29

Diversity of Archaea IV

378
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
378
Diversity of Archaea I01:30

Diversity of Archaea I

514
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
514
The Phosphorus Cycle01:21

The Phosphorus Cycle

43.5K
Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.
43.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Arterial calcification due to CD73 deficiency presenting with critical limb-threatening ischemia successfully treated with iliac endarterectomy and distal bypass.

Journal of vascular surgery cases and innovative techniques·2026
Same author

Midterm Outcomes of the Endurant Limb Fenestration With Internal Iliac Artery Repair and Enhanced Preservation (ELFIRE) Procedure.

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists·2026
Same author

Examining selection dynamics and limitations in multi-round protein selection of high diversity libraries.

Protein engineering, design & selection : PEDS·2026
Same author

Single-molecule kinetic exploration of functional sub-states in an evolving phosphotriesterase.

Nature communications·2026
Same author

Free energy perturbations in enzyme kinetic models reveal cryptic epistasis.

PLoS computational biology·2026
Same author

Cooperation and the evolution of bacterial niche breadth.

Proceedings of the National Academy of Sciences of the United States of America·2026

相关实验视频

Updated: Jan 9, 2026

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

16.3K

广泛的散乱的性酸酶强调了古老的微生物酸盐利用.

Morito Sakuma1, Naoki Konno2,3, Sevan Gholipour1

  • 1Michael Smith Laboratories, Faculty of Science, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Proceedings of the National Academy of Sciences of the United States of America
|December 4, 2025
PubMed
概括

细菌使用性酸酶 (PhoA) 将酸盐 (Pt) 氧化为酸盐,解决酸盐稀缺问题. 这种被忽视的PhoA功能在细菌中很普遍,对循环至关重要.

关键词:
性酸酶是一种性性酸酶.酸盐的酸盐是一种酸盐.酸盐氧化酶是一种酸盐氧化酶.的代谢是的代谢.

更多相关视频

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

9.1K
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
09:11

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Published on: November 23, 2019

7.1K

相关实验视频

Last Updated: Jan 9, 2026

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

16.3K
Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

9.1K
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
09:11

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Published on: November 23, 2019

7.1K

科学领域:

  • 微生物学 微生物学
  • 生物化学 生物化学
  • 环境科学 环境科学

背景情况:

  • 酸盐是一种重要的营养素,通常很稀缺,限制了细菌生长和生物分子的产生.
  • 细菌拥有酶,可以利用酸盐 (Pt) 等替代源来克服酸盐的限制.
  • 酸盐氧化中的特定酶和微生物作用及其对循环的影响尚未完全理解.

研究的目的:

  • 为了识别和描述与酸盐 (Pt) 氧化有关的细菌酶.
  • 研究Pt-氧化酶,特别是性酸酶 (PhoA) 的进化史和功能性乱交.
  • 阐明微生物Pt氧化在细菌酸盐代谢和全球循环中的作用.

主要方法:

  • 对Pt-氧化酶的生物信息分析,包括酸脱酶 (PtxD) 和酸酶 (PhoA).
  • 在细菌系中对PhoA和PtxD分布的比较进化分析.
  • 现存和重建的祖先PhoA酶对Pt氧化活性的生物化学表征.
  • 位点定向突变发生,以评估活性位点残留物对PhoA的Pt氧化酶和本源功能的影响.

主要成果:

  • 性酸酶 (PhoA) 在细菌中广泛分布,在进化的早期出现,而酸脱酶 (PtxD) 在特定的血统中后来出现.
  • 大多数测试的PhoA酶,包括祖先形式,都表现出酸盐氧化活性.
  • 破坏PhoA中的活性位点残留物降低了其酸盐氧化酶活性,只对其本源功能产生了部分影响.

结论:

  • 酸具有无序的酸盐氧化能力,代表了细菌酸获取的一个被忽视的机制.
  • 这种PhoA的杂乱功能在细菌酸盐代谢和生态系统中的酸盐循环中发挥着重要作用.
  • PhoA的广泛性表明它在各种环境中对微生物酸盐氧化有很大贡献.