在竹马赛克病毒-Dendrocalamus latiflorus相互作用中,N6-甲基氨酸和聚A) 尾部介导的转录后调节
Xiangrong Li1, Lin Wu2, Huihui Wang2
1Basic Forestry and Proteomics Research Center, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
The Plant journal : for cell and molecular biology
|December 4, 2025
概括
竹马赛克病毒 (BaMV) 感染通过改变蛋白质合成,光合作用和RNA处理来破坏Dendrocalamus latiflorus的生长. 这项研究揭示了受感染的竹子中关键的转录后调节机制.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 兰花 (D. latiflorus) 在经济上很重要,但易受竹马赛克病毒 (BaMV) 的感染.
- 在D. latiflorus.中,BaMV感染会导致显著的生长障碍.
- 控制这种相互作用的复杂分子机制,包括转录后调节,尚不清楚.
研究的目的:
- 为了阐明D. latiflorus在BaMV感染时的蛋白质和转录组变化.
- 调查转录后调节的作用,包括RNA处理和表体转录组修饰,在竹病毒相互作用中的作用.
- 确定关键的调节蛋白和参与植物对BaMV的反应的途径.
主要方法:
- 基于双重质量标签 (TMT) 的定量蛋白质组学来分析蛋白质表达特征.
- 纳米孔直接RNA测序 (DRS) 来评估转录组动态和RNA处理.
- 转录组分析以调查N6 - - 甲基氨酸 (m6A) 修改.
- 生物信息分析以整合蛋白质组和转录组数据.
主要成果:
- 在D. latiflorus中,BaMV感染抑制了与光合作用相关的蛋白质,同时提高了蛋白质合成,降解和抗氧化途径的调节.
- 鉴定出APR1蛋白质是连接硫代谢,抗氧化防御和光合作用的调节枢纽.
- 观察到减少全长RNA读取比率和改变多基化位点使用 (PR基因的远位点和更长的尾巴).
- 在POR和NCED1基因中增加的m6A修饰比与减少的转录相关,在BaMV基因组中确定了122个m6A位点.
结论:
- 转录后调节,包括RNA处理和m6A修改,在D. latiflorus对BaMV感染的反应中起着至关重要的作用.
- 改变的蛋白质组概况和转录组动态有助于疾病的发展和植物的防御.
- 了解这些监管网络为管理经济重要竹种的BaMV提供了洞察力.
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