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Updated: Jan 9, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

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无洗电化学生物传感器用于CpG甲基化,使用准直接电子转移类型酶作为标记酶

Mizuki Tomizawa1, Runa Hosaka2, Daimei Miura3

  • 1Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan; SilCreTech Co., Ltd., 3-59-10 Kameido, Koto, Tokyo, 136-0071, Japan.

Biosensors & bioelectronics
|December 4, 2025
PubMed
概括

我们开发了一种新的电化学检测系统,用于使用修改后的酶进行CpG甲基化. 这种无洗系统准确地量化了DNA甲基化水平从0-100%.

关键词:
氨基反应性纳乙硫酸盐通过CpG甲基化.葡萄糖脱水酶的使用甲基CpG结合域 甲基CpG结合域几乎是直接的电子转移.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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