在Danio rerio中对PFOA毒性的多个终点评估:氧化,基因毒性和代谢反应
Thais Braga Gomes Araujo1, Sidney Fernandes Sales Junior2, Gabriel de Farias Araujo2
1Programa de Pós-Graduação em Saúde Pública e Meio Ambiente, Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil; Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Journal of hazardous materials
|December 4, 2025
概括
像PFOA一样的Per-和多醇基物质 (PFAS) 在斑马鱼中引起显著的细胞损伤和代谢干扰,即使环境度低. 这突显了PFAS污染的生态风险和需要更严格的法规.
科学领域:
- 环境毒理学环境毒理学
- 生物化学 生物化学
- 生态毒理学 生态毒理学
背景情况:
- 和多基基物质 (PFAS) 是持久的环境污染物.
- 它们的生物积累和毒性引发了生态问题.
- 关于PFAS对水生生物的生化,遗传毒性和代谢作用的综合研究有限.
研究的目的:
- 评估PFOA对成年斑马鱼的多层次毒性影响.
- 为了将生物化学,基因毒性和代谢反应与PFOA暴露联系起来.
- 评估PFOA在环境相关度下的影响.
主要方法:
- 暴露成年斑马鱼在PFOA度范围 (0.01-1000μg/L) 的21天内.
- 对抗氧化剂防御生物标志物的分析 (catalase,谷氨酸S转移酶).
- 氧化和DNA损伤的评估.
- 代谢分析,以确定代谢途径的变化.
- 综合生物标志物响应 (IBRv2) 和主要成分分析 (PCA) 用于数据解释.
主要成果:
- 显著抑制了甲基酶和谷氨酸S转移酶.
- 显著增加脂质过氧化和DNA损伤,可检测到0.1μg/L的PFOA.
- IBRv2表明肝脏功能严重受损.
- PCA确定氧化应激是主要的毒性途径.
- 代谢学揭示了脂质,碳水化合物和氨基酸代谢的破坏,包括不和脂肪酸生物合成和银河糖/糖原路径.
结论:
- 暴露于PFOA会诱导斑马鱼的多层次毒性,影响抗氧化防御,导致DNA损伤,并破坏关键的代谢途径.
- 在环境相关的度下,微弱的PFOA暴露可能会危害鱼类的健康.
- 这些发现强调了PFAS污染的生态风险,并支持加强环境监测和监管.
相关概念视频
01:23Toxicity Testing in Animals
Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
01:28Microbial Bioremediation of Pesticides
Pesticides often feature structurally complex chemical architectures, incorporating halogen groups and multiple aromatic rings. These characteristics confer high chemical stability, rendering many pesticides resistant to natural degradation processes. This resistance poses significant environmental concerns, as persistent pesticide residues can accumulate in ecosystems and affect non-target organisms.Despite the inherent stability of many pesticides, certain microorganisms possess the metabolic...