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血小板表现出免疫表型变化和费城阴性骨髓增殖性新生瘤中失调的转录基因特征

Vitor Leonardo Bassan¹, Poliana Carina Paolini¹, Lilian Maria Garcia Ramos¹

¹Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

Thrombosis research

|December 4, 2025

概括

骨髓增殖性瘤 (MPN) 中的血小板是内在激活和转录性改变,导致血栓炎症和并发症. 这凸显了它们在MPN病变发生过程中的关键作用.

科学领域:

血液学血液学血液学
在瘤学瘤学.
免疫学免疫学免疫学

背景情况:

骨髓增殖性瘤 (MPN),如真多细胞血症,基本血栓血症和原发性骨髓纤维化,与JAK2,CALR和MPL突变有关.
慢性炎症是MPN的关键特征,推动疾病的进展和诸如血栓形成等并发症.
血小板变化可能会显著影响MPN中的血栓炎症状态.

研究的目的:

为了研究MPN患者的血小板免疫类型,活体激活和转录基因特征.
将这些特征与健康对照进行比较.
了解血小板在MPN相关的血栓炎症中的作用.

主要方法:

评估炎症指数:中性粒细胞与淋巴细胞的比率 (NLR),血小板与淋巴细胞的比率 (PLR) 和全身免疫炎症指数 (SII).
在基线和刺激后进行血小板免疫类型识别 (离子体A23187或血).
对照血小板暴露于MPN血;对失调路径的转录组数据的分析.

主要成果:

在MPN患者中,炎症指数升高 (NLR,PLR,SII).
MPN血小板显示了一个预激活的表型,具有增加的CD62P,CD36,CD63和CD154表达.
MPN血小板对血栓素的反应较弱;接触MPN血的对照血小板被激活. 转录组分析揭示了细胞骨组织,信号传递,粘附,新陈代谢和贩运中的下调基因.

结论:

血小板在MPN中是内在激活和转录失调,即使在治疗.
这些血小板异常是MPN中血栓炎症状态的关键贡献者.
血小板在MPN患者观察到的静血和血栓并发症中发挥着重要作用.

关键词:

流动细胞计量流动细胞计量炎症炎症是一种炎症.费城阴性骨髓扩散性瘤血小板是血小板的组成部分.血栓形成的原因之一是血栓形成.

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