核受体ERR通过对Nilaparvata lugens中的细胞染色体P450进行上调来促进伊米达克洛普里德耐药性
Woo-Ram Park1, Byungyoon Choi1, Nanthini Sadasivam2
1Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea; Host-Directed Antiviral Research Center, Chonnam National University, Gwangju, 61186, Republic of Korea.
Insect biochemistry and molecular biology
|December 4, 2025
概括
昆虫耐药性是一个重大挑战. 研究人员通过调节排毒基因,确定了与雌激素相关的受体 (NlERR) 对于棕色植物的伊米达克洛普里德耐药性至关重要.
科学领域:
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
- 遗传学 是一个遗传学.
背景情况:
- 像棕色植物 (Nilaparvata lugens) 这样的害虫对杀虫剂的耐药性是一个重要的农业问题.
- 核受体 (NRs) 调节代谢过程,但它们在N. lugens中的杀虫剂排毒中的作用尚不清楚.
研究的目的:
- 为了研究雌激素相关受体 (NlERR) 在N. lugens. 耐药性中的imidacloprid (IMD) 的作用.
- 为了确定由NlERR.R.调节的特定排毒基因.
主要方法:
- 对缺少NlERR的N. lugens.进行全基因组转录组分析.
- 在IMD治疗和NlERR淘汰后对细胞染色体P450 (CYP) 基因表达的分析.
- 染色体免疫沉 (ChIP) 证实NlERR与CYP基因促进体结合.
主要成果:
- 在IMD耐药的N. lugens菌株中,NlERR过度表达.
- NlERR与排毒基因转录具有正相关性,并直接与CYP4CE1促进体结合.
- NlERR倒置降低了IMD耐药性,并降低了向基因CYP4CE1和CYP6CW1.1的表达.
结论:
- NlERR是N. lugens. 中的伊米达克洛普里德耐药性的关键调节者.
- 针对NlERR提供了一种潜在的策略,以克服这种害虫的抗昆虫剂耐药性.
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