汉斯尼亚斯波拉物种可以在基于代谢学分析的三重菌株同时酒精-乳糖发酵过程中为Oenococcus oeni创造一个特权的利基
Yuzhu Zhao1, Dingyi Wang1, Kangjie Lou1
1College of Enology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Food microbiology
|December 4, 2025
概括
非Saccharomyces酵母,特别是Hanseniaspora物种,可显著加速乳发酵 (MLF) 超过50%并促进细菌生长. 它们通过创造代谢和为Oenococcus oeni.提供必需的营养物质来实现这一目标.
科学领域:
- 葡萄酒学 葡萄酒学 葡萄酒学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 众所周知,非Saccharomyces酵母在共同发酵期间可以提高乳发酵 (MLF) 的效率.
- 这些酵母增强MLF的确切机制在很大程度上是未知的.
研究的目的:
- 阐明特定非Saccharomyces酵母菌株提高MLF效率的机制.
- 为葡萄酒制造建立强大的三重菌株同时酒精-乳糖发酵系统.
主要方法:
- 三方发酵试验涉及16种非Saccharomyces酵母菌株,Saccharomyces cerevisiae F5和Oenococcus oeni SD-2a.
- 代谢分析用于研究代谢相互作用和营养利用.
- 对酵母衍生分泌和外源氨基酸补充的评估.
主要成果:
- 汉斯尼亚斯波拉 spp. 汉斯尼亚斯波拉 spp. 减少了50%的MLF持续时间,并增加了O. oeni生物量超过10倍.
- 欧尼尼利用马拉酸作为酵母形的中碳的来源,高调的尼古丁胺代谢促进了酶活性.
- 特定的酵母菌株分泌出支持细菌生长和新陈代谢的和代谢物 (酸盐,阿拉比托尔),而像谷氨酸这样的氨基酸进一步提高了效率.
结论:
- 特定的非Saccharomyces酵母,特别是汉氏菌,创造有利的代谢,显著加速MLF.
- 酵母驱动的糖竞争,尼古丁胺代谢和菌株特异性分泌是提高O. oeni性能的关键机制.
- 这项研究通过通过定义的微生物联盟优化同时酒精和乳发酵,为精密酒学奠定了基础.
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