由益生菌Bacillus subtilis CU1产生的Levan抑制了人类诺罗病毒GII.4在斑马鱼中的复制,通过高强度结合
Siyu Chen1, Malcolm Turk Hsern Tan2, Jillinda Yi Ling Toh2
1Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore; Department of Food Science and Technology, National University of Singapore (Suzhou) Research Institute, Suzhou, Jiangsu, China.
Food microbiology
|December 4, 2025
概括
来自Bacillus subtilis CU1的外聚糖体对人类诺罗病毒GII.4.4.表现出特定的抗病毒活性. 发酵的胡卜汁是生产这些功能益生菌外聚糖的具有成本效益的基质.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 生物化学 生物化学
背景情况:
- 人类诺病毒 (hNoVs) 是胃肠炎的重要原因.
- 益生菌细菌衍生的外聚糖 (EPSs) 正在探索抗病毒性质.
研究的目的:
- 调查益生菌细菌EPSs对hNoVs的抗病毒潜力.
- 确定针对hNoVs有效的特定EPS和细菌菌株.
- 探索可扩展的生产方法,以获得有前途的抗hNoV EPS.
主要方法:
- 使用图兰病毒 (TV) 和斑马鱼模型对来自Bacillus subtilis CU1,B. subtilis R0179和Lactiplantibacillus plantarum 299V的EPS进行查.
- 使用唾液结合ELISA和生物层干扰测量对活性EPS分量的表征.
- 用B. subtilis CU1发酵胡卜汁以产生和验证EPS抗病毒活性.
主要成果:
- 来自B. subtilis CU1的EPS对hNoV GII.4表现出特定的抗病毒活性,但对其他基因型或菌株没有.
- 积极的EPS被确定为一种具有高结合亲和力与hNoV GII.4病毒样颗粒的 levan.
- 发酵的胡卜汁促进了B. subtilis CU1的抗hNoV EPS的生产,以优化产量.
结论:
- 细菌细菌CU1衍生的外聚糖是对抗人类诺罗病毒GII.4.4.的有希望的药物.
- 胡卜汁发酵提供了一种安全,具有成本效益和可扩展的方法来生产功能益生菌EPSs.
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