质蛋白 OsCPIP8 通过抑制致病蛋白酶和激活大米免疫来提供双重保护
Jian Zhang1,2,3, Chongchong Lu1,2,3, Yihan Sun1,2,3
1State Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai' an, China.
Nature communications
|December 4, 2025
概括
米囊蛋白酶抑制剂 OsCPIP8 阻止病原体 XoCysP1 降解防御蛋白 OsXIP2.2. OsCPIP8还触发了植物免疫力,增强了大米对Xanthomonas oryzae pv.的抵抗力. 子球 (Xoc) 感染.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 生物化学 生物化学
背景情况:
- 氨酸蛋白酶在植物免疫力中至关重要,但由病原体分泌的蛋白酶会破坏宿主防御.
- 涉及宿主-病原体蛋白酶相互作用的病原机制在细胞中尚未完全理解.
- 克桑托莫纳斯里扎 (Xanthomonas oryzae pv.) 是一个植物. 桃菌 (Xoc) 是一个重要的米病原体.
研究的目的:
- 阐明大米抵抗Xoc的分子机制,重点关注蛋白酶相互作用.
- 为了确定涉及到"攻击和防御"相互作用的关键蛋白质,在米的apoplast.
- 探索开发新型生物农药和耐药作物品种的潜在目标.
主要方法:
- 研究了Xoc分泌的氨酸蛋白酶 XoCysP1和大米氨酸酶抑制蛋白 OsXIP2.2 之间的相互作用.
- 分析了米囊蛋白酶抑制蛋白 OsCPIP8 在调节 XoCysP1 活性和 OsXIP2 稳定性的作用.
- 研究了受体类激酶OsRLK8和OsSERK2在OsCPIP8介导的免疫信号传递中的参与.
主要成果:
- XoCysP1降解了米的防御蛋白 OsXIP2,从而损害了米对Xoc的抵抗力.
- OsCPIP8与 XoCysP1 相互作用,抑制 OsXIP2 的降解并赋予耐药性.
- OsCPIP8被OsRLK8和OsSERK2识别,从而激活下游植物的免疫反应.
结论:
- OsCPIP8作为一种双重功能蛋白,保护OsXIP2并激活大米免疫力.
- 这项研究揭示了一种新的"攻击和防御"途径,涉及蛋白酶抑制和受体介导免疫.
- 这些发现为设计增强农作物抗菌病原体的基础.
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