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针对外表面蛋白C的多价mRNA疫苗提供了针对莱姆病的多菌株保护

Annabelle Pfeifle^1,2, Casey Lansdell¹, Wanyue Zhang^1,2

¹Centre for Oncology, Radiopharmaceuticals and Research, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada and World Health Organization Collaborating Center for Standardization and Evaluation of Biologicals, Ottawa, ON, Canada.

|December 4, 2025

概括

开发一种针对外表面蛋白C (OspC) 的mRNA疫苗显示了莱姆病预防的前景. 一个多价疫苗证明了对某些Borrelia burgdorferi菌株的保护,支持进一步开发广泛的莱姆病覆盖范围.

科学领域:

疫苗学疫苗学疫苗学
微生物学微生物学
免疫学免疫学免疫学

背景情况:

目前没有人类莱姆病 (LD) 疫苗存在.
波雷莉亚甲虫的外表面蛋白C (OspC) 是LD疫苗的关键目标.
在OspC的遗传多样性使广泛保护疫苗的开发复杂化.

研究的目的:

开发和评估编码OspC变异的单价和多价mRNA疫苗.
评估由OspC向的mRNA疫苗诱导的保护疗效和免疫反应.

主要方法:

开发一种单价mRNA疫苗 (OspC类型A) 和一种多价mRNA疫苗 (OspC类型A,C,I,K,N).
通过同源和异源的挑战模型在小鼠中测试疫苗的疗效.
对OspC特异性抗体和CD4+T细胞反应的分析.

主要成果:

单价疫苗提供了对同源挑战的完整保护.
多价疫苗诱导了针对所有目标OspC类型的抗体.
观察到对表达OspC类型A,I和K的Borrelia burgdorferi菌株的保护,但没有C或N.
较高的疫苗剂量改善了对OspC型C菌株的保护.

结论:

这项研究首次成功展示了针对莱姆病的OspC向的mRNA疫苗.
基于OspC的mRNA疫苗具有广泛的莱姆病预防潜力.
为了实现全面的保护,可能需要进一步优化多价配方.

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