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针对儿科慢性病诊断的非向的唾液代谢

Nora Unceta^1,2, Alicia Sánchez-Ortega³, Alberto Gómez-Caballero^4,5

¹Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain. nora.unceta@ehu.eus.

Scientific reports

|December 4, 2025

概括

唾液生物标志物可以帮助诊断儿科慢性病 (CKD) 非侵入性. 代谢学分析确定了特定的唾液化合物,可以准确地预测儿童的CKD,为血液测试提供了一个有希望的替代方案.

科学领域:

生物化学生化学
代谢学代谢学代谢学
儿科脏病学儿科脏病学

背景情况:

慢性病 (CKD) 对儿童造成重大健康风险,需要早期检测.
目前的诊断方法通常依赖于侵入性血液检测.
开发非侵入性生物标志物对于在儿科患者中及时诊断CKD至关重要.

研究的目的:

确定潜在的唾液生物标志物,用于早期,非侵入性诊断儿科CKD.
为了比较来自非刺激与酸刺激的唾液的代谢资料的诊断效用.
建立唾液代谢学作为儿科CK诊断的可行工具.

主要方法:

使用液体染色学-四极点飞行时间质谱法 (LC-QTOF-MS) 的非目标代谢学.
分析了儿童慢性病患者和健康对照的非刺激和酸刺激的唾液样本.
统计分析包括部分最小方程方法-区分分析 (PLS-DA) 和接收器运行特征 (ROC) 曲线.

主要成果:

在CKD患者和对照人群之间,在未受刺激和受刺激的唾液中观察到不同的代谢概况.
特定的生物标志物 (prolylproline,leucyl-leucine,pyroglutamyl-leucine) 在CKD患者的未刺激的唾液中升高.
克reatine,n-butyrylcarnitine和palmitic acid在CKD患者刺激的唾液中发生了变化.
与未受刺激的唾液 (AUC=0.874) 相比,刺激的唾液生物标志物显示出更高的诊断准确性 (AUC=0.999).

结论:

唾液代谢学,特别是使用刺激样本,显示出非侵入性儿科CKD诊断的高潜力.
唾液采集的方法对已确定的代谢概况和诊断准确性有重大影响.
唾液代谢组分析是早期检测儿童CKD的一个有希望的工具.

关键词:

酸性刺激是一种酸性刺激.慢性脏疾病慢性脏疾病儿科儿科儿科这是唾液的唾液.没有针对性的代谢组分.

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