在生物样本中同时和灵敏地量化蛋白质和低分子量硫化物,聚硫化物和H2S
Jan Lj Miljkovic1, Nils Burger1, Chak Shun Yu1
1Medical Research Council-Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK.
Nature communications
|December 4, 2025
概括
硫化 (H2S) 修改了硫醇,影响了抗氧化防御. 这项研究引入了一种敏感的LC-MS/MS方法来量化这些醇修饰,进步了我们对生物固硫化的理解.
科学领域:
- 生物化学 生化学
- 分析化学 分析化学
- 细胞生物学 细胞生物学
背景情况:
- 硫化 (H2S) 在细胞过程中发挥着至关重要的作用,通过可逆地修改.
- 了解H2S修饰,包括硫化物 (RSS−) 和多硫化物 (RS(S) nS−),对于抗氧化剂防御和活性调节至关重要.
- 目前在量化这些变化的局限性阻碍了生物学意义的探索.
研究的目的:
- 开发一种敏感的分析方法来量化生物样本中的H2S变化.
- 为了能够同时确定低分子量 (LMW) 和蛋白质 (Pr) 硫化物和多硫化物.
- 探索受化影响的生物系统.
主要方法:
- 开发一种敏感的液体染色学-并联质谱法 (LC-MS/MS) 程序.
- 捕获硫原子以产生H2S,RSS−和RS(S) nS−的诊断产品.
- 在同一样本中同时量化LMW和Pr硫化物/多硫化物.
- 使用谷氨 (GSH) 量化验证测量结果的直角方法.
主要成果:
- 一种新的LC-MS/MS方法成功量化了H2S,硫化物和多硫化物.
- 该方法允许同时确定LMW和Pr thiol的修饰.
- 在各种生物系统中得到了应用,从蛋白质到ex vivo组织.
结论:
- 开发的方法为探索生物系统中的化提供了一个敏感的工具.
- 这一进步有助于更深入地了解H2S介导的生物过程.
- 能够对生物化学和细胞生物学中的关键氧化还原变化进行定量分析.
相关概念视频
Qualitative Analysis
For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
For instance, group IV...
Preparation of Samples for Electron Microscopy
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Sample Preparation for Analysis: Advanced Techniques
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...


