暴露于氨酸,2,4-二甲基氨酸和2-甲基氨酸的工人的综合性尿代谢物概况
Yoko Eitaki1, Makiko Nakano1,2, Kazuyuki Omae1
1Department of Preventive Medicine and Public Health, Keio University School of Medicine, Tokyo, Japan.
Journal of occupational health
|December 5, 2025
概括
这项研究确定了关键的尿路代谢物,用于监测职业接触芳香胺,如氨酸. 这些发现表明,尿液中的特定化合物总量是有效的生物暴露标记物.
科学领域:
- 职业健康 职业健康 职业健康
- 毒理学 毒理学 毒理学
- 分析化学 分析化学
背景情况:
- 生物暴露监测对于评估危险物质的皮肤吸收至关重要.
- 目前用于芳胺的方法经常忽略代谢物,限制了准确性.
- 改善监测需要分析母化合物及其代谢物.
研究的目的:
- 量化尿液中不变芳胺及其代谢物的度.
- 为了确定不同尿排泄产品的比例.
- 建立可靠的生物标志物,用于职业接触氨酸,m-xylidine和o-toluidine.
主要方法:
- 收集了11名暴露于芳香胺的工人的下班尿样.
- 使用了酶性水解 (葡萄糖酶和硫酶) 预处理.
- 使用液体染色学-并联质谱法量化了25种物质.
主要成果:
- 主要的尿路代谢物包括化和N-乙化化合物,以及侧链氧化产物.
- 发现少量的不变和N-乙化化合物.
- 在人体和动物模型中,新陈代谢和分泌模式不同.
结论:
- 不变的化合物和主要代谢物的总和可以作为监测生物暴露的可行标记.
- 特定的标志物组合被确定为氨酸,2,4-二甲基氨酸和2-甲基氨酸暴露.
- 建议在连续工作日进行轮班结束尿样采集,以进行精确的监测.
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