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相关概念视频

RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jan 9, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
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Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

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异形:用于转录级RNA-seq解释的工作流.

Izabela Mamede1,2, Lucio R Queiroz1,3, Carlos Mata-Machado1

  • 1Laboratory of Genetics Biochemistry, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.

NAR genomics and bioinformatics
|December 5, 2025
PubMed
概括
此摘要是机器生成的。

Isoformic是一个新的R管道,用于在转录层面分析RNA测序数据. 它有助于揭示传统基因水平分析遗漏的复杂基因表达模式,揭示生物相关的转录变异.

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

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相关实验视频

Last Updated: Jan 9, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
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Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 转录组分析在现代生物学中至关重要,但往往忽视了替代拼接和转录启动/终止.
  • 大多数哺乳动物基因 (超过95%) 产生多个转录,但短读RNA测序 (RNA-seq) 分析与转录级解读扎.
  • 现有的工具缺乏准确性,注释的一致性和高效的下游生物解释能力.

研究的目的:

  • 介绍Isoformic,一个可定制的R管道,用于对短读RNA-seq数据的转录级分析.
  • 为了能够检测转录级别的变化,可视化外子-内子结构,并根据转录类型分层的功能丰富分析.
  • 提高RNA-seq研究的分辨率,并揭示替代转录事件的调节作用.

主要方法:

  • 开发一个可定制的R管道,命名为Isoformic.
  • 处理微分表达结果以识别转录级别的变化.
  • 使用各种数据集进行验证,包括孕前,SARS-CoV-2感染和小鼠焦虑模型.
  • 与GENCODE参考转录组的兼容性.

主要成果:

  • 异形成功检测出具有转录水平变化的基因.
  • 管道可视化外子-内子结构,并执行转录类型分层的功能丰富.
  • 经过验证的研究揭示了生物相关的转录变异及其潜在的表型关联.
  • 异形增强了RNA-seq数据的解释性.

结论:

  • Isoformic提供了一个强大的解决方案,用于对短读RNA-seq数据的转录级分析.
  • 该管道克服了现有工具的局限性,提供了更好的准确性和生物洞察力.
  • 异形授权研究人员探索替代转录事件的功能意义.