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Updated: Jan 9, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
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微流体辅助精子分类器:一种高通量精子选择装置.

Dhiraj B Puri1, Vadiraj Hemadri1, Gautam Biswas1

  • 1Department of Mechanical Engineering, Birla Institute of Technology and Science, Pilani, K K Birla Goa Campus, Zuarinagar, Sancoale, Goa-403726, India. siddharthat@goa.bits-pilani.ac.in.

Lab on a chip
|December 5, 2025
PubMed
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一种新的微流体装置有效地使用自然选择原理对精子细胞进行分类,显著提高了运动性,活力,并减少了辅助生殖技术 (ART) 的DNA损伤. 这种方法提高了IVF和ICSI等程序的精子质量.

科学领域:

  • 生殖生物学 生殖生物学
  • 生物医学工程 生物医学工程
  • 微流体学 微流体学

背景情况:

  • 不孕症是一个全球性的健康问题,辅助生殖技术 (ART) 的成功取决于精子选择.
  • 传统方法可能会损害精子DNA,对临床结果产生负面影响.
  • 模仿自然选择障碍对于高质量的精子隔离至关重要.

研究的目的:

  • 开发一种新的,经济高效的微流体辅助精子分类 (MASS) 装置.
  • 通过使用自然选择机制,实现运动精子细胞的高通量分离.
  • 评估排序精子细胞的生存能力和DNA完整性.

主要方法:

  • 设计了一种两层的微流体装置,利用精子转移和边界遵循行为.
  • 采用不同的流速来引导运动精子到采集室.
  • 进行数值模拟以优化流动动力学和生物测试以评估精子质量.

主要成果:

  • MASS装置在20分钟内从500μL精液中分离出超过250万个运动精子/毫升,效率为8.1%.
  • 分类精子显示了98.83%的运动性,98.2%的活力和2.84%的DNA碎片化.
  • 在精子质量指标上明显优于传统的上游方法.

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Last Updated: Jan 9, 2026

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结论:

  • 该MASS设备提供了一种优越的方法,以获得高质量的精子用于试管婴儿和ICSI.
  • 它提供了足够数量的活力精子,而DNA损伤最小.
  • 该设备是研究精子动力学和动学的宝贵工具.