Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

PCR01:32

PCR

236.8K
Overview
236.8K
Real Time RT-PCR02:57

Real Time RT-PCR

64.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Recent Advances in Vision-Based Beef Cattle Body Measurement Technologies.

Animals : an open access journal from MDPI·2026
Same author

Diagnostic performance of machine learning models based on dual-phase 99mTc-MIBI SPECT/CT semiquantitative parameters for differentiating benign and malignant pulmonary nodules.

PloS one·2026
Same author

Polymer-Gated Bilayer Buccoadhesive Tablets for Biphasic Release of Indomethacin: Balancing Dissolution and Mucoadhesion.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Discovery and validation of a prognostic SPP1/PLAU signature in HPV-negative oropharyngeal squamous cell carcinoma.

BMC medical genomics·2026
Same author

Rosmarinic acid ameliorates radiation-induced intestinal injury through PI3K/AKT/NF-κB/MMP9 axis inhibition: From network pharmacology prediction to experimental validation.

Tissue & cell·2026
Same author

The mechanisms of myricetin and quercetin in regulating miRNA-140 and MMP/TIMP signaling pathway in osteoarthritis treatment.

Pakistan journal of pharmaceutical sciences·2026

相关实验视频

Updated: Jan 9, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection
09:02

Rapid PCR Thermocycling using Microscale Thermal Convection

Published on: March 5, 2011

23.3K

一个以试剂为中心的热控制系统,由高速PCR级联温度控制算法驱动.

Yuheng Luo1,2,3, Wangyang Hu1,2, Jiajia Wu3

  • 1National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangzhou, 510641, China. pmrdd@scut.edu.cn.

The Analyst
|December 5, 2025
PubMed
概括

这项研究引入了一个快速定量聚合酶连锁反应 (qPCR) 平台,可以在不到5分钟内实现45个周期的放大. 新的试剂为中心的控制策略确保了高速核酸测试,而不会牺牲准确性.

更多相关视频

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

7.5K
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.4K

相关实验视频

Last Updated: Jan 9, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection
09:02

Rapid PCR Thermocycling using Microscale Thermal Convection

Published on: March 5, 2011

23.3K
Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

7.5K
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.4K

科学领域:

  • 分子诊断学 分子诊断学
  • 生物技术是生物技术.
  • 控制系统工程 控制系统工程

背景情况:

  • 加快定量聚合酶连锁反应 (qPCR) 对于分子诊断至关重要,但受到热滞后的阻碍.
  • 现有的方法难以平衡速度与分析准确性.

研究的目的:

  • 开发一个高速的qPCR平台,使用一种新的控制策略克服热滞.
  • 在不影响定量准确性的情况下实现快速的核酸放大.

主要方法:

  • 采用了以试剂为中心的级联控制策略,使用虚拟温度传感器.
  • 一个基于平面PCB的铜加热器作为加热元件和传感器.
  • 模糊的PID控制与进料补偿调节的试剂温度.

主要成果:

  • 试剂的加热和冷却速度达到24.1°C/s和19°C/s.
  • 控制试剂温度,准确度为±0.2°C,超标<0.2°C.
  • 在4.4分钟内完成了45个周期的放大,R2=0.9965和109.8%的效率.

结论:

  • 高速的qPCR平台展示了快速而准确的核酸测试.
  • 试剂为中心的控制框架可用于高通量分子诊断.
  • 这种方法可以实现更快,更高效的分子诊断仪器.