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通过电子喷射,用氨酸功能化的酸微球为CD44介导的益生菌的向输送
Wenhao Ding1, Fengzhi Qiao1, Shichao Xuan1
1State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Journal of agricultural and food chemistry
|December 5, 2025
概括
这项研究开发了针对Clostridium butyricum使用氨酸修饰的酸微球进行向的益生菌输送. 这种方法增强了细菌的生存和结肠向,有效治疗结肠炎.
科学领域:
- 生物材料工程 生物材料工程
- 胃肠病学 胃肠病学
- 微生物学 微生物学
背景情况:
- 益生菌输送在维持细菌活力和在胃肠道内实现向输送方面面临挑战.
- 有效的传递系统对于最大限度地发挥益生菌的治疗潜力至关重要,如Clostridium butyricum (CB).
研究的目的:
- 开发一种针对Clostridium butyricum (CB) 的向输送系统,使用用酸酸 (HA) 修改的酸微球 (ALG MSs).
- 在大肠炎模型中研究HA-修饰的CB-装载酸微球 (CBHA@ALGHA MSs) 的增强粘附性,向积累和治疗疗效.
主要方法:
- 使用电喷技术制造包装Clostridium butyricum的酸盐微球.
- 用氨酸对微球和细菌进行修改,以实现CD44受体介导的向.
- 在体外评估封装效率,细菌存活率和细胞粘附.
- 使用光成像和DSS诱导的大肠炎小鼠模型进行体内评估.
主要成果:
- CBHA@ALGHA MSs显示了高封装效率 (69.23%) 和消化后存活率 (94.56%).
- HA修饰显著增强了对炎症Caco-2细胞的粘附性,并在炎症结肠部位的向积累 (增加1.7倍).
- 口服CBHA@ALGHA MSs缓解了DSS诱导的大肠炎,增加了短链脂肪酸,恢复了肠道屏障的完整性,并改善了微生物平衡.
结论:
- 酸对酸微球的修饰使得Clostridium butyricum能够有针对性地向炎症的结肠组织输送.
- 这一策略提高了益生菌的生存能力,提高了结肠炎的治疗疗效,并为先进的益生菌应用提供了有前途的方法.
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