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Updated: Jan 9, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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FLARE:一种无标签的光辅助方法,用于RNA工程的三向连接

A Murali Krishna1, Nida Fathima1, Jothi Basu2

  • 1Department of Chemistry, Indian Institute of Science Education and Research (IISER), Tirupati 517619, India. a.sharma@iisertirupati.ac.in.

Organic & biomolecular chemistry
|December 5, 2025
PubMed
概括

我们开发了FLARE,一种用于设计RNA三向结 (3WJs) 的新方法,以提高稳定性和功能. 这种方法显著提高了RNA的稳定性,在CRISPR-Cas9和纳米医学中有应用.

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Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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