在LPS诱导的炎症模型中,慢性社会压力下的肠道微生物群和免疫反应
Pavlo Petakh1, Yaroslav Stravskyy2, Iryna Halabitska3
1Department of Biochemistry and Pharmacology, Uzhhorod National University, Uzhhorod, Ukraine.
Stress (Amsterdam, Netherlands)
|December 5, 2025
概括
慢性社会压力改变了对质脂多糖 (LPS) 的免疫反应,并显著改变了大鼠的肠道微生物群组成. 这些发现突出了压力,炎症和肠道微生物组之间的相互作用.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 压力研究 压力研究
背景情况:
- 慢性心理压力会影响免疫功能和肠道微生物群.
- 压力对质脂多糖 (LPS) 诱导的炎症的影响尚不清楚.
研究的目的:
- 研究慢性社会压力如何影响LPS诱导的全身炎症的老鼠的免疫基因表达和肠道微生物群.
- 探索压力,炎症和微生物变化之间的关系.
主要方法:
- 小鼠接受了LPS注射和/或慢性社会压力 (过度拥挤).
- 免疫基因表达被分析使用RT2 分析器PCR阵列.
- 用基于培养的方法评估肠道微生物群组成.
- 统计分析包括t测试,克鲁斯卡尔-瓦利斯,PCA和斯皮尔曼相关性.
主要成果:
- LPS诱导了六个免疫基因 (Il6,Il1b,Ifng,Il10,CCL3,CCL5) 的显著上调.
- 压力减少了某些基因 (Il10,Tnf) 的LPS诱导的上调.
- 在各组之间观察到不同的肠道微生物群,特定细菌的丰度发生了变化 (例如,Bifidobacterium,Lactobacillus,Klebsiella).
- 在Klebsiella spp.之间发现了积极的相关性. 和Il1b之间,以及在压力较大的群体中Alcaligenes faecalis和Tnf之间.
结论:
- 慢性社会压力调节了对LeptoSpiral LPS的免疫反应.
- 压力显著改变了肠道微生物群的组成.
- 微生物群-免疫相互作用可能会调解压力诱导的系统性炎症变化.
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