Cas9调节Campylobacter jejuni在肠道上皮细胞内部的毒性特征
Chinmoy Saha1,2, Dior Beerens1, Peter van Baarlen3
1Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center Rotterdam, Rotterdam, Netherlands.
Microbiology (Reading, England)
|December 5, 2025
概括
来自Campylobacter jejuni的CRISPR相关蛋白9 (Cas9) 通过影响细胞外完整性和抗生素耐药性来增强细菌的毒性. 这种Cas9蛋白显著提高了人体细胞的细胞内生存率,突出显示了它在C. jejuni感染中的作用.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是细菌性胃肠炎的主要原因之一.
- 来自致病细菌的CRISPR相关蛋白9 (Cas9) 越来越多地被认为是其在毒性中的作用.
- 在宿主-病原体相互作用中,C. jejuni Cas9 (CjeCas9) 的特定功能仍然不完全理解.
研究的目的:
- 调查CjeCas9在C. jejuni细胞包裹完整性,抗生素耐药性和细胞内生存中的作用.
- 确定CjeCas9对Caco-2肠上皮细胞入侵和托尔类受体2 (TLR-2) 激活的影响.
主要方法:
- 对C. jejuni cas9.9的基因删除突变结构.
- 评价细胞外的透性和脂质醇糖的表达.
- 抗生素敏感性测试 (西普罗夫洛克萨).
- 在Caco-2细胞中量化细胞内细菌存活率.
- 测量TLR-2激活的情况.
主要成果:
- CjeCas9显著调节了C. jejuni细胞包膜的透性和化脂氧糖体的表达.
- CjeCas9会影响对西普罗夫洛克萨的敏感性,西普罗夫洛克萨是治疗C. jejuni感染的关键抗生素.
- 野生型C. jejuni表达CjeCas9的Caco-2细胞内细胞内存活率比cas9删除突变体增加了550倍.
- 细胞内生存与TLR-2激活相关.
结论:
- CjeCas9是C. jejuni. 的一个关键的毒性因子.
- CjeCas9增强了宿主肠道上皮细胞内的细菌细胞内生存.
- 通过TLR-2激活,CjeCas9有助于通过TLR-2激活免疫逃避,这代表了一个潜在的治疗标.
相关概念视频
Bacterial Translocation and Protein Secretion
471
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
471
cAMP-dependent Protein Kinase Pathways
8.2K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.2K
Gene Regulation in Microbial Communities: Quorum Sensing
473
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
473
Stringent Response in E. coli
267
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
267
Mucosal Barrier of the Stomach
2.0K
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
2.0K
CRISPR
57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K


