相关实验视频

Updated: Jan 9, 2026

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

来自传染性病病毒分离物UPM1432/2019的VP2蛋白的化分析:结构动态和表位预测

Ali Youssif Mansour^1,2, Abdul Rahman Omar^3,2, Mohd Hair Bejo^3,2

¹Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Antonie van Leeuwenhoek

|December 5, 2025

概括

传染性病病毒 (IBDV) 对家禽构成威胁. 这项研究分析了VP2蛋白质,确定了稳定的区域和潜在的表位,用于开发新的IBDV热稳定疫苗.

科学领域:

兽医病毒学兽医病毒学
结构生物学结构生物学
免疫信息学是指免疫信息学.

背景情况:

传染性病病毒 (IBDV) 是主要的家禽病原体,导致免疫抑制和经济损失.
高IBDV突变率需要新的疫苗策略,特别是热稳定的疫苗.

研究的目的:

通过计算分析马来西亚IBDV分离物的VP2蛋白 (UPM1432/2019).
为了阐明IBDV VP2蛋白的结构稳定性和抗原潜力.
为了确定下一代基于表位素的疫苗的潜在目标.

主要方法:

对VP2序列的遗传学分析.
对热稳定性和细胞稳定性进行物理化学分析.
在不同温度 (20°C至80°C) 的分子动力学模拟.
B细胞表位预测 (线性和构造性).

主要成果:

VP2蛋白具有很高的热稳定性 (阿利法特指数:98.77) 和细胞稳定性 (不稳定性指数:17.55).
高温逐渐破坏蛋白质结构的稳定,由增加的RMSD和减少的结合证明.
确定了12个线性和7个构造性B细胞表位,其中保留了表面暴露的区域,显示出高抗原性.
一种高度免疫主导的形状表位 (L168-E181) 显示出显著的免疫识别潜力 (ElliPro评分:0.903).

结论:

IBDV VP2 蛋白质具有稳定且免疫反应的域.
计算洞察力支持开发热稳定,以表位基的IBDV控制疫苗.
了解VP2结构-抗原性对于对抗家禽IBDV至关重要.

关键词:

抗原性是一种抗原性.皮层预测预测的预测分子动力学模拟模型蛋白质的稳定性蛋白质的稳定性这是一种VP2蛋白质.

更多相关视频

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

相关概念视频

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...