相关实验视频

Updated: Jan 9, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

靠近红外的MINFLUX成像是通过抑制光体闪而实现的

Chinmaya V Srambickal¹, Hanie M Esmaeeli¹, Joachim Piguet¹

¹Experimental Biomolecular Physics, Bio-Opto-Nano Unit, Department of Applied Physics, KTH, Royal Institute of Technology, SE-10691 Stockholm, Sweden.

Science advances

|December 5, 2025

概括

研究人员通过研究光体闪来改进极小光子FLUXes (MINFLUX) 超高分辨率显微镜. 开发了战略,使近红外MINFLUX成像具有纳米精度.

科学领域:

生物物理学的生物物理.
光学显微镜的使用方法
纳米技术纳米技术

背景情况:

最小光子FLUXes (MINFLUX) 显微镜可以达到纳米精度.
低光体要求是相对于其他超分辨率显微镜 (SRM) 技术的优势.
局限性包括局部化概率低和使用不太明亮/光稳定光体的困难,特别是近红外 (NIR) 光体.

研究的目的:

克服MINFLUX成像中的局限性,特别是关于光体稳定性和定位概率的局限性.
为了使近红外 (NIR) 光体在MINFLUX.中使用.
为MINFLUX和一般的SRM开发优化样品和激发条件的策略.

主要方法:

系统地研究深红色和近红外光线氨酸光体的闪特性.
在各种条件下 (时间尺度,样本/刺激参数) 模拟MINFLUX局部化.
研究光的闪机制 (光异构化,光还原) 和错误来源.

主要成果:

通过光异构化和光还原的光体闪被确定为局部化错误的主要原因.
平衡的氧化还原缓冲器和重复激发束扫描有效地抑制眼引起的错误.
证明了成功的NIR-MINFLUX成像与纳米定位精度.

结论:

开发了一些策略来缓解光体闪,并提高MINFLUX中的定位精度.
启用了NIR-MINFLUX成像,扩大了超分辨率显微镜的工具包.
为优化MINFLUX和其他SRM技术中的成像条件提供了一个一般框架.

更多相关视频

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.